Rabbit anti-Rat PAI-1 Polyclonal Antibody | anti-SerpinE1 antibody

Anti-PAI-1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8113020

• Gene Names: Serpine1; Pai1; PAI1A; Planh; Pai1aa; RATPAI1A
• Reactivity: Rat
• Applications: ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: PAI-1; Polyclonal Antibody; Anti-PAI-1 Antibody; Rabbit Polyclonal; PAI-1/SerpinE1 Antibody; Rabbit PAb; Antigen Affinity Purified; serpin peptidase inhibitor; clade E (nexin; plasminogen activator inhibitor type 1); member 1; Anti-Pai1 Antibody; Anti-Planh1 Antibody; Anti-SERPINE1 Antibody; anti-SerpinE1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rat PAI-1/SerpinE1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SerpinE1 antibody: ELISA (EIA)
• Application Notes: ELISA: 1:5000-1:10,000
• Immunogen: Recombinant Rat PAI-1/SerpinE1 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Rat PAI-1/SerpinE1 (rR PAI-1/SerpinE1). PAI-1/SerpinE1 specific IgG was purified by Rat PAI-1/SerpinE1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-SerpinE1 antibody

Plasminogen activator inhibitor 1, also known as PAI-1, Endothelial plasminogen activator inhibitor, SerpinE1 and PLANH1, is a secreted glycoprotein that belongs to the serpin family. SerpinE1 is the primary physiological inhibitor of the two plasminogen activators urokinase (uPA) and tissue plasminogen activator (tPA). Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Defects in SerpinE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency) which is characterized by abnormal bleeding due to SerpinE1 defect in the plasma. High concentrations of SerpinE1 have been associated with thrombophilia which is an autosomal dominant disorder in which affected individuals are prone to develop serious spontaneous thrombosis. Studies of PAI-1 have contributed significantly to the elucidation of the protease inhibitory mechanism of serpins, which is based on a metastable native state becoming stabilised by insertion of the RCL into the central beta-sheet A and formation of covalent complexes with target proteases. Greater expression of PAI-1 has been associated with increased survival of cells and resistance to apoptosis. PAI-1 appears to influence apoptosis by decreasing cell adhesion (anoikis) as well as its effect on intracellular signaling. PAI-1, in its active state, also binds to the extracellular protein vitronectin. When in complex with its target proteases, it binds with high affinity to endocytosis receptors of the low density receptor family. The mechanisms of PAI-1 overexpression during obesity are complex, and it is conceivable that several inducers are involved at the same time at several sites of synthesis. PAI-1 is also implicated in adipose tissue development. It suggests that PAI-1 inhibitors serve in the control of atherothrombosis.

References

Alessi MC, et al. (2006) PAI-1 and the metabolic syndrome: links, causes, and consequences. Arterioscler Thromb Vasc Biol. 26(10): 2200-7.Schneider DJ, et al. (2008) The effect of plasminogen activator inhibitor type 1 on apoptosis. Thromb Haemost. 100(6): 1037-40.Dupont DM, et al. (2009) Biochemical properties of plasminogen activator inhibitor-1. Front Biosci. 14: 1337-61.

NCBI and Uniprot Product Information

• NCBI GI #: 6981332
• NCBI GeneID: 24617
• NCBI Accession #: NP_036752.1
• NCBI GenBank Nucleotide #: NM_012620.1
• UniProt Accession #: P20961
• Molecular Weight: 160,000
• NCBI Official Full Name: plasminogen activator inhibitor 1
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
• NCBI Official Symbol: Serpine1
• NCBI Official Synonym Symbols: Pai1; PAI1A; Planh; Pai1aa; RATPAI1A
• NCBI Protein Information: plasminogen activator inhibitor 1; PAI; PAI-1; serpin E1; endothelial plasminogen activator inhibitor; serine (or cysteine) proteinase inhibitor, member 1; serine (or cysteine) peptidase inhibitor, clade E, member 1; serine (or cysteine) proteinase inhibi
• UniProt Protein Name: Plasminogen activator inhibitor 1
• Protein Family: Plasminogen activator inhibitor
• UniProt Gene Name: Serpine1
• UniProt Synonym Gene Names: Pai1; Planh1; PAI; PAI-1
• UniProt Entry Name: PAI1_RAT

NCBI Description

mediates inhibition of fibrinolysis by inhibiting the activity of plasminogen activator; may promote neuronal survival [RGD, Feb 2006]

Uniprot Description

SERPINE1: a secreted protein that acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protease binding; receptor binding

Biological Process: positive regulation of blood coagulation; wound healing; response to lipopolysaccharide; positive regulation of coagulation; decidualization; negative regulation of smooth muscle cell migration; glucose homeostasis; defense response to Gram-negative bacterium; mast cell activation; positive regulation of receptor-mediated endocytosis; positive regulation of interleukin-8 production; positive regulation of fibroblast proliferation; negative regulation of fibrinolysis; tissue regeneration; angiogenesis; regulation of receptor activity; chronological cell aging; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; female gonad development; negative regulation of proteolysis; positive regulation of keratinocyte migration; regulation of angiogenesis; regulation of cell proliferation; response to starvation; response to reactive oxygen species; positive regulation of angiogenesis; response to hyperoxia; cellular response to insulin stimulus; response to estrogen stimulus; response to cytokine stimulus; positive regulation of inflammatory response