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Western Blot (WB) (Western Blot analysis of extracts of various cell lines, using AGPAT1 antibody.)

Rabbit AGPAT1 Polyclonal Antibody | anti-AGPAT1 antibody

AGPAT1 Polyclonal Antibody

Gene Names
AGPAT1; G15; LPAATA; 1-AGPAT1; LPAAT-alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
AGPAT1; Polyclonal Antibody; AGPAT1 Polyclonal Antibody; 1-acyl-sn-glycerol-3-phosphate; acyltransferase alpha; 1-acylglycerol-3-phosphate O-acyltransferase 1; 1-AGP acyltransferase 1; 1-AGPAT 1; Lysophosphatidic acid acyltransferase alpha; LPAAT-alpha; Protein G15; G15; anti-AGPAT1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
AGPAT1 polyclonal antibody detects endogenous levels of AGPAT1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
283
Applicable Applications for anti-AGPAT1 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB 1:500-1:2000
IP 1:50-1:100
Immunogen
Recombinant full length Human AGPAT1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using AGPAT1 antibody.)

Western Blot (WB) (Western Blot analysis of extracts of various cell lines, using AGPAT1 antibody.)
Related Product Information for anti-AGPAT1 antibody
Phosphatidic acid and lysophosphatidic acid are phospholipids involved in lipid biosynthesis and signal transduction. LPAAT-alpha (lysophosphatidic acid acyltransferase alpha), also designated 1-acylglycerol-3-phosphate O-acyltransferase 1 (AGPAT1), catalyzes the synthesis of phosphatidic acid from lysophosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. LPAAT-alpha is a membrane-bound protein belonging to the LPAAT family. Members of the LPAAT family have a well-known role in lipid biosynthesis and may also play a role in tumor progression. LPAAT-alpha is widely expressed with highest expression in skeletal muscle.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 32kDa
NCBI Official Full Name
1-acyl-sn-glycerol-3-phosphate acyltransferase alpha isoform 1
NCBI Official Synonym Full Names
1-acylglycerol-3-phosphate O-acyltransferase 1
NCBI Official Symbol
AGPAT1
NCBI Official Synonym Symbols
G15; LPAATA; 1-AGPAT1; LPAAT-alpha
NCBI Protein Information
1-acyl-sn-glycerol-3-phosphate acyltransferase alpha
UniProt Protein Name
1-acyl-sn-glycerol-3-phosphate acyltransferase alpha
UniProt Gene Name
AGPAT1
UniProt Synonym Gene Names
G15; 1-AGP acyltransferase 1; 1-AGPAT 1; LPAAT-alpha
UniProt Entry Name
PLCA_HUMAN

NCBI Description

This gene encodes an enzyme that converts lysophosphatidic acid (LPA) into phosphatidic acid (PA). LPA and PA are two phospholipids involved in signal transduction and in lipid biosynthesis in cells. This enzyme localizes to the endoplasmic reticulum. This gene is located in the class III region of the human major histocompatibility complex. Alternative splicing results in two transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Research Articles on AGPAT1

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Product Notes

The AGPAT1 agpat1 (Catalog #AAA3007829) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AGPAT1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AGPAT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB 1:500-1:2000 IP 1:50-1:100. Researchers should empirically determine the suitability of the AGPAT1 agpat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AGPAT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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