Rabbit anti-Human, Mouse HMGCS1 Polyclonal Antibody | anti-HMGCS1 antibody

HMGCS1 (3-hydroxy-3-methylglutaryl Coenzyme A Synthase, Hydroxymethylglutaryl-CoA Synthase Cytoplasmic, HMG-CoA Synthase, HMGCS, MGC90332) (MaxLight 405)

Cat. Num. AAA6481013

• Gene Names: HMGCS1; HMGCS
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: HMGCS1; Polyclonal Antibody; HMGCS1 (3-hydroxy-3-methylglutaryl Coenzyme A Synthase; Hydroxymethylglutaryl-CoA Synthase Cytoplasmic; HMG-CoA Synthase; HMGCS; MGC90332) (MaxLight 405); 3-hydroxy-3-methylglutaryl-CoA Synthase 1 (Soluble); EC=2.3.3.10; anti-HMGCS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HMGCS1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-HMGCS1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human HMGCS1 (KLH).
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HMGCS1 antibody (Center) in HL60 cell line lysates (35ug/lane). HMGCS1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of HMGCS1 antibody (Center) in mouse liver tissue lysates (35ug/lane). HMGCS1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HMGCS1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of hela cells using HMGCS1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-HMGCS1 antibody

Antibodies; Coenzyme A

References

1. Vock, C., Cell. Physiol. Biochem. 22(5-6), 515-524 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 148298764
• NCBI GeneID: 3157
• NCBI Accession #: NP_002121
• NCBI GenBank Nucleotide #: NM_002130
• UniProt Accession #: Q01581
• Molecular Weight: 57kDa
• NCBI Official Full Name: hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
• NCBI Official Synonym Full Names: 3-hydroxy-3-methylglutaryl-CoA synthase 1
• NCBI Official Symbol: HMGCS1
• NCBI Official Synonym Symbols: HMGCS
• NCBI Protein Information: hydroxymethylglutaryl-CoA synthase, cytoplasmic
• UniProt Protein Name: Hydroxymethylglutaryl-CoA synthase, cytoplasmic
• Protein Family: Hydroxymethylglutaryl-CoA synthase
• UniProt Gene Name: HMGCS1
• UniProt Synonym Gene Names: HMGCS; HMG-CoA synthase
• UniProt Entry Name: HMCS1_HUMAN