Rabbit p70S6K Polyclonal Antibody | anti-p70S6K antibody

p70S6K Rabbit Polyclonal

Cat. Num. AAA7602323

• Gene Names: RPS6KB1; S6K1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: p70S6K; Polyclonal Antibody; p70S6K Rabbit Polyclonal; anti-p70S6K antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 527

• Applicable Applications for anti-p70S6K antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Immunogen: Ribosomal protein S6 kinase, 70kDa, polypeptide 1
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using MBS7602323 (p70 (S6K) Antibody) at dilution of 1:200)

Immunofluorescence (IF)

(Immunofluorescent analysis of HepG2 cells using MBS7602323 (p70S6K Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG)

Western Blot (WB)

(HeLa cells were subjected to SDS PAGE followed by western blot with MBS7602323 (p70 (S6K) antibody) at dilution of 1:1200)

NCBI and Uniprot Product Information

• NCBI GI #: 37702835
• NCBI GeneID: 404181
• Molecular Weight: 59,352 Da
• NCBI Official Full Name: p70S6K
• NCBI Official Symbol: RPS6KB1
• NCBI Official Synonym Symbols: S6K1
• NCBI Protein Information: ribosomal protein S6 kinase beta-1
• UniProt Protein Name: Ribosomal protein S6 kinase beta-1
• UniProt Gene Name: RPS6KB1
• UniProt Synonym Gene Names: S6K-beta-1; S6K1; P70S6K1; p70-S6K 1
• UniProt Entry Name: KS6B1_BOVIN

Uniprot Description

Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the preinitiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR ().