Western Blot (WB)
(Western blot analysis of p35 expression in rat brain (A); HeLa (B); A431 (C) whole cell lysates.)
Rabbit anti-Mouse, Rat p35 Polyclonal Antibody | anti-p35 antibody
p35 Antibody
Gene Names
CDK5R1; p23; p25; p35; CDK5R; NCK5A; CDK5P35; p35nck5a
Reactivity
Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Immunogen Affinity Chromatography
Synonyms
p35; Polyclonal Antibody; p35 Antibody; Purified Polyclonal p35 Antibody; Polyclonal p35 antibody; Anti-p35 antibody; CDK5R antibody; NCK5A antibody; Cyclin-dependent kinase 5 activator 1 antibody; CDK5 activator 1 antibody; Cyclin-dependent kinase 5 regulatory subunit 1 antibody; TPKII regulatory subunit antibody; anti-p35 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of p35 protein
Purity/Purification
Immunogen Affinity Chromatography
Form/Format
Supplied in liquid form in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3 with 30% Glycerol and 0.01% Sodium Azide
Sequence Length
307
Applicable Applications for anti-p35 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1:500-1:1000
IF: 1:100-1:500
ICC: 1:100-1:500
IF: 1:100-1:500
ICC: 1:100-1:500
Immunogen
p35 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human p35 as the immunogen
Cross-Reactivity
Mouse, Rat, B
Preparation and Storage
Store at 4 degree C for short term storage. For long term, aliquot and store at -20 degree C. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of p35 expression in rat brain (A); HeLa (B); A431 (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of p35 expression in rat brain (A); HeLa (B); A431 (C) whole cell lysates.)
Related Product Information for anti-p35 antibody
p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling.
Product Categories/Family for anti-p35 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
p35
NCBI Official Synonym Full Names
cyclin dependent kinase 5 regulatory subunit 1
NCBI Official Symbol
CDK5R1
NCBI Official Synonym Symbols
p23; p25; p35; CDK5R; NCK5A; CDK5P35; p35nck5a
NCBI Protein Information
cyclin-dependent kinase 5 activator 1
Protein Family
NCBI Description
The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease. [provided by RefSeq, Jul 2008]
Research Articles on p35
Similar Products
Product Notes
The p35 (Catalog #AAA5315271) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p35 Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's p35 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1:500-1:1000 IF: 1:100-1:500 ICC: 1:100-1:500. Researchers should empirically determine the suitability of the p35 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p35, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
