Rat Nuclear Receptor Coactivator 3 (NCOA3) RTU ELISA Kit | NCOA3 rtu elisa kit

Rat Nuclear Receptor Coactivator 3 (NCOA3) ELISA Kit

Cat. Num. AAA4501306

• Gene Names: Ncoa3; Aib1; Tram-1
• Reactivity: Rat
• Synonyms: Nuclear Receptor Coactivator 3 (NCOA3); Rat Nuclear Receptor Coactivator 3 (NCOA3) ELISA Kit; RAC3; ACTR; AIB1; CAGH16; CTG26; SRC3; TNRC14; TNRC16; TRAM1; pCIP; Amplified In Breast 1; Steroid Receptor Coactivator-3; Thyroid Hormone Receptor Activator Molecule 1; NCOA3 rtu elisa kit

• Reactivity: Rat
• Sequence Length: 1082

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.115ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NCOA3 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NCOA3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NCOA3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NCOA3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NCOA3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 23396767
• NCBI GeneID: 84584
• UniProt Accession #: Q9EPU2
• Molecular Weight: 115,134 Da
• NCBI Official Full Name: Nuclear receptor coactivator 3
• NCBI Official Synonym Full Names: nuclear receptor coactivator 3
• NCBI Official Symbol: Ncoa3
• NCBI Official Synonym Symbols: Aib1; Tram-1
• NCBI Protein Information: nuclear receptor coactivator 3
• UniProt Protein Name: Nuclear receptor coactivator 3
• Protein Family: Nuclear receptor coactivator
• UniProt Gene Name: Ncoa3
• UniProt Synonym Gene Names: Aib1; NCoA-3; AIB-1

NCBI Description

stimulates transcriptional activities of nuclear receptors [RGD, Feb 2006]

Uniprot Description

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, probably via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit ().