Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human heart tissue using MBS7133640 at dilution of 1:100)
Rabbit anti-Human, Rat TNNI3 Polyclonal Antibody | anti-TNNI3 antibody
TNNI3 Antibody
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
IF: 1:200-1:500
IP: 1:200-1:2000
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human heart tissue using MBS7133640 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human heart tissue using MBS7133640 at dilution of 1:100)
Immunoprecipitation (IP)
(Immunoprecipitating TNNI3 in Rat heart tissueLane 1: Rabbit control IgG instead of MBS7133640 in Rat heart tissue.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000)Lane 2: MBS7133640 (8ug) + Rat heart tissue (500ug)Lane 3: Rat heart tissue (20ug))
Immunoprecipitation (IP)
(Immunoprecipitating TNNI3 in Rat heart tissueLane 1: Rabbit control IgG instead of MBS7133640 in Rat heart tissue.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000)Lane 2: MBS7133640 (8ug) + Rat heart tissue (500ug)Lane 3: Rat heart tissue (20ug))
Immunofluorescence (IF)
(Immunofluorescence staining of Hela cells with MBS7133640 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of Hela cells with MBS7133640 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
NCBI and Uniprot Product Information
NCBI Description
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]
Uniprot Description
TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.
Protein type: Motility/polarity/chemotaxis; Motor; Actin-binding
Chromosomal Location of Human Ortholog: 19q13.4
Cellular Component: sarcomere; troponin complex; cytosol
Molecular Function: troponin T binding; protein domain specific binding; troponin C binding; protein binding; metal ion binding; calcium channel inhibitor activity; actin binding; protein kinase binding; calcium-dependent protein binding
Biological Process: regulation of smooth muscle contraction; cellular calcium ion homeostasis; heart contraction; regulation of systemic arterial blood pressure by ischemic conditions; heart development; ventricular cardiac muscle morphogenesis; vasculogenesis; negative regulation of ATPase activity; muscle filament sliding; cardiac muscle contraction
Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Familial Restrictive, 1; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Dilated, 2a
Research Articles on TNNI3
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Product Notes
The TNNI3 tnni3 (Catalog #AAA7133640) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TNNI3 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TNNI3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). IHC: 1:20-1:200 IF: 1:200-1:500 IP: 1:200-1:2000. Researchers should empirically determine the suitability of the TNNI3 tnni3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNNI3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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