Rabbit anti-Human OXCT1 Polyclonal Antibody | anti-OXCT1 antibody

Anti-OXCT1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8109153

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: OXCT1; Polyclonal Antibody; Anti-OXCT1 Antibody; Rabbit Polyclonal; OXCT1 Antibody; Rabbit PAb; Antigen Affinity Purified; 3-oxoacid CoA transferase 1; Anti-OXCT Antibody; Anti-SCOT Antibody; anti-OXCT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human OXCT1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-OXCT1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:200-1:1000; ICC/IF: 1:100-1:500; IP: 1-5uL/mg of lysate
• Immunogen: E Coli-derived Human OXCT1 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human OXCT1 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-OXCT1 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:56 kDa Observed band size:55 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human OXCT1 in human stomach with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of OXCT1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human OXCT1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

Immunoprecipitation (IP)

(OXCT1 was immunoprecipitated using: Lane A:0.5 mg U251MG Whole Cell Lysate 4 uL anti-OXCT1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-OXCT1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 56 kDa Observed band size :56 kDa)

Related Product Information for anti-OXCT1 antibody

Succinyl-CoA:3-oxoacid CoA transferase (SCOT, gene symbol OXCT1) deficiency is an autosomal recessive disorder in ketone body utilization that results in severe recurrent ketoacidosis episodes in infancy, including neonatal periods. 3-oxoacid CoA-transferase 1 (OXCT1) is a key enzyme in ketone body metabolism that catalyzes the first and rate-determining step of ketolysis and is expressed in adipose and other tissues. The product of OXCT1 converts to acetyl-CoA and finally fed into the tricarboxylic acid cycle for oxidation and ATP production. In the mitochondrial matrix, the OXCT1 gene catalyzes the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate in a reaction related to energy production from ketone bodies. OXCT1 may play a role in adipogenesis and provides new insight on adipose deposition in sheep.

References

Zhang S, et al. (2017) The role of oxct1 in the pathogenesis of cancer as a rate-limiting enzyme of ketone body metabolism. Life Sci 183 110-115.Sasai H, et al. (2017) Heterozygous carriers of succinyl-coa:3-oxoacid coa transferase deficiency can develop severe ketoacidosis. J Inherit Metab Dis 40 (6): 845-852.Nam GH, et al. (2012) Identification of orf sequences and exercise-induced expression change in thoroughbred horse oxct1 gene. Gene 496 (1): 45-48.