Myosin IA (MYO1A) Recombinant Protein | MYO1A recombinant protein

Recombinant Myosin IA (MYO1A)

Cat. Num. AAA2009844

• Gene Names: Myo1a; Myhl; BBM-I
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Myosin IA (MYO1A); Recombinant Myosin IA (MYO1A); MYO1A recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- MAQKFLLN LKKNLPSTKV LDNTWPAAPY RCFNTANQEL QRLFYQWKCK KFRDQLSPKQ VQTLREKLCA SELFKGKKAS YPQSVPIPFR GDYIGLQGNP KLQRLKGREE GPVLVADTVK KVNRGNGKTS ARILLLTKGH VILTDAKKSQ AQIVIGLEDV AGVSVSSLQD GLFSLHLSEM SSAVSKGDIL LVSDHVVELL TKMYQAVLDA TQRQLSVTVT EKFSVRFKEG SVAVKVIQGP EGGGNRKLIC KKKGSNAMEV TVR
• Sequence Length: 1043

• Applicable Applications for MYO1A recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Met783~Arg1043 (Accession # O88329) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 124487037
• NCBI GeneID: 432516
• NCBI Accession #: NP_001074688.1
• NCBI GenBank Nucleotide #: NM_001081219.2
• UniProt Accession #: O88329; B2RW65
• Molecular Weight: 30.4kDa
• NCBI Official Full Name: unconventional myosin-Ia
• NCBI Official Synonym Full Names: myosin IA
• NCBI Official Symbol: Myo1a
• NCBI Official Synonym Symbols: Myhl; BBM-I
• NCBI Protein Information: unconventional myosin-Ia; BBMI; MIHC; myosin I heavy chain; brush border myosin 1; brush border myosin I; myosin, heavy polypeptide-like (110kD)
• UniProt Protein Name: Unconventional myosin-Ia
• Protein Family: Unconventional myosin
• UniProt Gene Name: Myo1a
• UniProt Synonym Gene Names: Bbmi; Myhl; BBM-I; BBMI; MIHC
• UniProt Entry Name: MYO1A_MOUSE

Uniprot Description

MYO1A: Involved in directing the movement of organelles along actin filaments (Potential). Defects in MYO1A are the cause of deafness autosomal dominant type 48 (DFNA48). DFNA48 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

Protein type: Motility/polarity/chemotaxis; Actin-binding; Motor

Cellular Component: neuron projection; cortical actin cytoskeleton; microvillus; basolateral plasma membrane; leading edge; lipid raft; actin cytoskeleton; filamentous actin; growth cone; cell soma; cytoplasm; apical plasma membrane; basal plasma membrane; myosin complex; lateral plasma membrane; brush border

Molecular Function: calmodulin binding; actin filament binding; nucleotide binding; motor activity; actin binding; ATP binding

Biological Process: microvillus biogenesis; sensory perception of sound; regulation of protein localization; cell projection organization and biogenesis; vesicle localization