Human OSBPL1A ELISA Kit | OSBPL1A elisa kit

Human OSBPL1A (Oxysterol Binding Protein Like Protein 1A) ELISA Kit

Cat. Num. AAA2516076

• Gene Names: OSBPL1A; ORP1; ORP-1; OSBPL1B
• Reactivity: Human
• Synonyms: OSBPL1A; Human OSBPL1A (Oxysterol Binding Protein Like Protein 1A) ELISA Kit; OSBPL1A elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinantHumanOSBPL1A. No significant cross-reactivity or interference between HumanOSBPL1A and analogues was observed.
• Sequence Length: 568

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OSBPL1A elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanOSBPL1Aconcentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to OSBPL1A. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for OSBPL1Aand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain OSBPL1A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of OSBPL1A.You can calculate the concentration of OSBPL1Ain the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 335353788
• NCBI GeneID: 114876
• NCBI Accession #: NP_001229437.1
• NCBI GenBank Nucleotide #: NM_001242508.1
• Molecular Weight: 65,095 Da
• NCBI Official Full Name: oxysterol-binding protein-related protein 1 isoform 3
• NCBI Official Synonym Full Names: oxysterol binding protein-like 1A
• NCBI Official Symbol: OSBPL1A
• NCBI Official Synonym Symbols: ORP1; ORP-1; OSBPL1B
• NCBI Protein Information: oxysterol-binding protein-related protein 1; OSBP-related protein 1; oxysterol binding protein-like 1B; oxysterol-binding protein-related protein 1 variant 1; oxysterol-binding protein-related protein 1 variant 2
• UniProt Protein Name: Oxysterol-binding protein-related protein 1
• Protein Family: Oxysterol-binding protein-related protein
• UniProt Gene Name: OSBPL1A
• UniProt Synonym Gene Names: ORP1; OSBP8; OSBPL1; OSBPL1B; ORP-1; OSBP-related protein 1
• UniProt Entry Name: OSBL1_HUMAN

NCBI Description

This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. Transcript variants derived from alternative promoter usage and/or alternative splicing exist; they encode different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

OSBPL1A: Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3- phosphate. Stabilizes GTP-bound RAB7A on late endosomes/lysosomes and alters functional properties of late endocytic compartments via its interaction with RAB7A. Belongs to the OSBP family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 18q11.1

Cellular Component: nucleoplasm; lysosomal membrane; late endosome; cytoplasm; intracellular; nucleus

Molecular Function: protein binding; cholesterol binding; phospholipid binding

Biological Process: vesicle-mediated transport; cholesterol metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class II; lipid transport