Rabbit anti-Human OGDH Polyclonal Antibody | anti-OGDH antibody

OGDH, CT (OGDH, 2-oxoglutarate dehydrogenase, mitochondrial, 2-oxoglutarate dehydrogenase complex component E1, Alpha-ketoglutarate dehydrogenase) (APC)

Cat. Num. AAA6327646

• Gene Names: OGDH; E1k; OGDC; AKGDH
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: OGDH; Polyclonal Antibody; CT (OGDH; 2-oxoglutarate dehydrogenase; mitochondrial; 2-oxoglutarate dehydrogenase complex component E1; Alpha-ketoglutarate dehydrogenase) (APC); Alpha-ketoglutarate dehydrogenase; anti-OGDH antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 1023

• Applicable Applications for anti-OGDH antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: OGDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-410 amino acids from the C-terminal region of human OGDH.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(OGDH Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the OGDH antibody detected the OGDH protein (arrow).)

Immunohistochemistry (IHC)

(OGDH Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of OGDH Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-OGDH antibody

This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Product Categories/Family for anti-OGDH antibody

Antibodies; Mitochondrial Proteins

References

van Bever, Y., et al. Am. J. Med. Genet. A 143(7):763-767(2007). Shi, Q., et al. J. Biol. Chem. 280(12):10888-10896(2005). Habelhah, H., et al. J. Biol. Chem. 279(51):53782-53788(2004). McCartney, R.G., et al. J. Biol. Chem. 273(37):24158-24164(1998). Koike, K. Gene 159(2):261-266(1995).

NCBI and Uniprot Product Information

• NCBI GI #: 51873036
• NCBI GeneID: 4967
• NCBI Accession #: NP_002532.2
• NCBI GenBank Nucleotide #: NP_002532.2
• UniProt Accession #: Q02218
• NCBI Official Full Name: 2-oxoglutarate dehydrogenase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: oxoglutarate dehydrogenase
• NCBI Official Symbol: OGDH
• NCBI Official Synonym Symbols: E1k; OGDC; AKGDH
• NCBI Protein Information: 2-oxoglutarate dehydrogenase, mitochondrial
• UniProt Protein Name: 2-oxoglutarate dehydrogenase, mitochondrial
• UniProt Gene Name: OGDH
• UniProt Synonym Gene Names: OGDC-E1
• UniProt Entry Name: ODO1_HUMAN

NCBI Description

This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]

Uniprot Description

OGDH: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). Belongs to the alpha-ketoglutarate dehydrogenase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - lysine degradation; Oxidoreductase; Carbohydrate Metabolism - citrate (TCA) cycle; Mitochondrial; EC 1.2.4.2

Chromosomal Location of Human Ortholog: 7p14-p13

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial membrane; cytosol; oxoglutarate dehydrogenase complex

Molecular Function: chaperone binding; metal ion binding; heat shock protein binding; thiamin pyrophosphate binding; oxoglutarate dehydrogenase (succinyl-transferring) activity

Biological Process: generation of precursor metabolites and energy; glycolysis; striatum development; succinyl-CoA metabolic process; tricarboxylic acid cycle; hippocampus development; pyramidal neuron development; thalamus development; 2-oxoglutarate metabolic process; cellular metabolic process; cerebellar cortex development; tangential migration from the subventricular zone to the olfactory bulb; NADH metabolic process; lysine catabolic process

Disease: Alpha-ketoglutarate Dehydrogenase Deficiency