Western Blot (WB)
(OGDH Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the OGDH antibody detected the OGDH protein (arrow).)
Rabbit anti-Human OGDH Polyclonal Antibody | anti-OGDH antibody
OGDH Antibody (C-term)
Western Blot (WB)
(OGDH Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the OGDH antibody detected the OGDH protein (arrow).)
Western Blot (WB)
(OGDH Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the OGDH antibody detected the OGDH protein (arrow).)
Immunohistochemistry (IHC)
(OGDH Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of OGDH Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(OGDH Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of OGDH Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
dehydrogenase complex. This complex catalyzes the overall
conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA
and CO(2) during the Krebs cycle. The protein is located in the
mitochondrial matrix and uses thiamine pyrophosphate as a cofactor.
A congenital deficiency in 2-oxoglutarate dehydrogenase activity is
believed to lead to hypotonia, metabolic acidosis, and
hyperlactatemia. Alternative splicing results in multiple
transcript variants encoding distinct isoforms.
Shi, Q., et al. J. Biol. Chem. 280(12):10888-10896(2005)
Habelhah, H., et al. J. Biol. Chem. 279(51):53782-53788(2004)
McCartney, R.G., et al. J. Biol. Chem. 273(37):24158-24164(1998)
Koike, K. Gene 159(2):261-266(1995)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]
Uniprot Description
OGDH: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). Belongs to the alpha-ketoglutarate dehydrogenase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - lysine degradation; Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.2.4.2; Oxidoreductase; Mitochondrial
Chromosomal Location of Human Ortholog: 7p14-p13
Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial membrane; oxoglutarate dehydrogenase complex; cytosol
Molecular Function: heat shock protein binding; metal ion binding; chaperone binding; thiamin pyrophosphate binding; oxoglutarate dehydrogenase (succinyl-transferring) activity
Biological Process: generation of precursor metabolites and energy; glycolysis; striatum development; succinyl-CoA metabolic process; tricarboxylic acid cycle; hippocampus development; pyramidal neuron development; thalamus development; 2-oxoglutarate metabolic process; cerebellar cortex development; cellular metabolic process; tangential migration from the subventricular zone to the olfactory bulb; NADH metabolic process; lysine catabolic process
Disease: Alpha-ketoglutarate Dehydrogenase Deficiency
Research Articles on OGDH
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Product Notes
The OGDH ogdh (Catalog #AAA9203561) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 381-410. The OGDH Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's OGDH can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the OGDH ogdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OGDH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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