anti-Human MeCP2 Antibody | anti-MeCP2 antibody

MeCP2 Antibody

Cat. Num. AAA8502236

• Gene Names: MECP2; RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
• Reactivity: Human
• Applications: Western Blot
• Synonyms: MeCP2; MeCP2 Antibody; anti-MeCP2 antibody

• Reactivity: Human
• Specificity: MeCP2 rabbit polyclonal antibody detects endogenous MeCP2 proteins (~53KD).
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 393

• Applicable Applications for anti-MeCP2 antibody: WB: 1:2, 000-5, 000
• Application Notes: Western Blot (WB)
• Preparation and Storage: Store at-20 degree C for 1 year

Testing Data

(Western blot analysis of Hela cell lysate with MeCP2 rabbit pAb diluted at 1:2,000 (lane 1) and 1:5,000 (lane 2).)

Related Product Information for anti-MeCP2 antibody

Methyl-CpG-binding protein 2(MeCP2), whose exact function is unclear. It appears to help regulate gene activity (expression) by modifying chromatin, the complex of DNA and protein that packages DNA into chromosomes. The MeCP2 protein usually regulates genes involved in brain function, even though this protein is found throughout the body.Within the brain, the MeCP2 protein is important for the function of nerve cells (neurons) and is present in high levels in mature neurons. This protein likely plays a role in maintaining connections (synapses) between neurons, where cell-to-cell communication occurs. Many of the genes that are known to be regulated by the MeCP2 protein play a role in normal brain function, particularly the maintenance of synapses.

NCBI and Uniprot Product Information

• NCBI GI #: 938320031
• NCBI GeneID: 4204
• NCBI Accession #: NP_001303266.1
• NCBI GenBank Nucleotide #: NM_001316337.1
• UniProt Accession #: P51608; O15233; Q6QHH9; Q7Z384
• Molecular Weight: 53,323 Da
• NCBI Official Full Name: methyl-CpG-binding protein 2 isoform 3
• NCBI Official Synonym Full Names: methyl-CpG binding protein 2
• NCBI Official Symbol: MECP2
• NCBI Official Synonym Symbols: RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
• NCBI Protein Information: methyl-CpG-binding protein 2
• UniProt Protein Name: Methyl-CpG-binding protein 2
• Protein Family: Methyl-CpG-binding protein
• UniProt Gene Name: MECP2
• UniProt Synonym Gene Names: MeCp-2 protein; MeCp2

NCBI Description

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of cognitive disability in females. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2015]

Uniprot Description

Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).