Rabbit Oct-1 Polyclonal Antibody | anti-Oct-1 antibody

Oct-1 Polyclonal Antibody

Cat. Num. AAA3012170

• Gene Names: POU2F1; OCT1; OTF1; oct-1B
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: ProA affinity purified
• Synonyms: Oct-1; Polyclonal Antibody; Oct-1 Polyclonal Antibody; OTF-1; POU2F1; OCT1; OTF1; FLJ42836; NF A1; NF-A1; Oct 1; Oct 1B; Octamer binding protein 1; Octamer binding transcription factor 1; Octamer-binding protein 1; Octamer-binding transcription factor 1; OTF 1; OTTHUMP00000032348; OTTHUMP00000032350; OTTHUMP00000032351; PO21; PO2F1; POU class 2 homeobox 1; POU domain class 2 transcription factor 1; POU domain; class 2; transcription factor 1; anti-Oct-1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Oct-1 polyclonal antibody detects endogenous levels of Oct-1 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 755

• Applicable Applications for anti-Oct-1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2,000; IHC: 1:50-1:200
• Immunogen: Recombinant protein within human Oct-1 aa 1-150.
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Oct-1 on SH-SY-5Y cell (1) and A431 cell (2) lysate using anti-Oct-1 antibody at 1/1,000 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-Oct-1 antibody. Counter stained with hematoxylin.)

Related Product Information for anti-Oct-1 antibody

POU domain proteins contain a bipartite DNA binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. The POU dimer from the Oct-1 gene formed on the palindromic Oct factor-recognition element (PORE), which is comprised of an inverted pair of homeodomain-binding sites separated by exactly 5 bp (ATTTGAAATGCAAAT), could recruit the transcriptional co-activator OBF1. Studies of tissue-specific expression of immunoglobulin promoters demonstrate the importance of an octamer, ATTTGCAT, and the proteins that bind to it. This is a regulatory element important for tissue- and cell-specific transcription, as well as for transcription of a number of housekeeping genes. The Oct-1 gene encodes one protein, NF-A1, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, which is encoded by the Oct-2 gene.

NCBI and Uniprot Product Information

• NCBI GI #: 311771667
• NCBI GeneID: 5451
• NCBI Accession #: NP_001185712.1
• NCBI GenBank Nucleotide #: NP_001185712.1
• UniProt Accession #: P14859; P25425; P31503
• Molecular Weight: Predicted Band Size: 76kDa
• NCBI Official Full Name: POU domain, class 2, transcription factor 1 isoform 2
• NCBI Official Synonym Full Names: POU class 2 homeobox 1
• NCBI Official Symbol: POU2F1
• NCBI Official Synonym Symbols: OCT1; OTF1; oct-1B
• NCBI Protein Information: POU domain, class 2, transcription factor 1
• UniProt Protein Name: POU domain, class 2, transcription factor 1
• UniProt Gene Name: POU2F1
• UniProt Synonym Gene Names: OCT1; OTF1; Oct-1; OTF-1
• UniProt Entry Name: PO2F1_HUMAN

NCBI Description

The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM, Jul 2010]

Uniprot Description

Oct1: a ubiquitous transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR. Phosphorylation apparently inhibits its interaction with DNA. Four splice variant isoforms have been described. Isoform 2 is lymphocyte specific.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 1q24.2

Cellular Component: nucleoplasm; transcription factor complex; intracellular membrane-bound organelle; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase III promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; olfactory placode formation