Western Blot (WB)
(Western Blot detection against Immunogen (107.47kD).)
Mouse anti-Human POU2F1 Monoclonal Antibody | anti-POU2F1 antibody
POU2F1 (POU Domain, Class 2, Transcription Factor 1, NF-A1, Octamer-binding Protein 1, Oct-1, Octamer-binding Transcription Factor 1, OTF-1, OCT1, OTF1) (AP)
Gene Names
POU2F1; OCT1; OTF1; oct-1B
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
POU2F1; Monoclonal Antibody; POU2F1 (POU Domain; Class 2; Transcription Factor 1; NF-A1; Octamer-binding Protein 1; Oct-1; Octamer-binding Transcription Factor 1; OTF-1; OCT1; OTF1) (AP); anti-POU2F1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
1E12
Specificity
Recognizes human POU2F1.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-POU2F1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to aa1-744 from human POU2F1 (AAH01664) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MNNPSETSKPSMESGDGNTGTQTNGLDFQKRPVPVGGAISTAQAQAFLGHLHQVQLAGTSLQAAAQSLNVQSKSNEESGDSQQPSQPSQQPSVQAAIPQTQLMLAGGQITGLTLTPAQQQLLLQQAQAQAQLLAAAVQQHSASQQHSAAGATISASAATPMTQIPLSQPIQIAQDLQQLQQLQQQNLNLQQFVLVHPTTNLQPAQFIISQTPQGQQGLLQAQNLLTQLPQQSQANLLQSQPSITLTSQPATPTRTIAATPIQTLPQSQSTPKRIDTPSLEEPSDLEELEQFAKTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLEKWLNDAENLSSDSSLSSPSALNSPGIEGLSRRRKKRTSIETNIRVALEKSFLENQKPTSEEITMIADQLNMEKEVIRVWFCNRRQKEKRINPPSSGGTSSSPIKAIFPSPTSLVATTPSLVTSSAATTLTVSPVLPLTSAAVTNLSVTGTSDTTSNNTATVISTAPPASSAVTSPSLSPSPSASASTSEASSASETSTTQTTSTPLSSPLGTSQVMVTASGLQTAAAAALQGAAQLPANASLAAVAAAAGLNPSLMAPSQFAAGGALPSLNPGTLSGALSPALMSNSTLATIQALASGGSLPITSLDATGNLVFANAGGAPNIVTAPLFLNPQNLSLLTSNPVSLVSAAAASAGNSAPVASLHATSTSAESIQNSLFTVASASGAASTTTTASKAQ
Conjugate
AP
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (107.47kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (107.47kD).)
Related Product Information for anti-POU2F1 antibody
OCT1/OTF1 is a protein belonging to the POU domain of transcription factors. OCT1, Octamer binding transcription factor 1, binds to an octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes for immunoglobulins. POU domain proteins contain a bipartite DNA-binding domain, which is divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is conferred at the dimerization level. POU dimer from the OCT1 gene formed on the palindromic OCT factor recognition element, or PORE, could recruit the transcriptional coactivator OBF1. This octamer sequence within immunoglobulin promoters are sufficient for lymphoid-specific promoter activity in lymphoid cells. OCT1 regulate the expansion and/or maintenance of mature B cells. In its absence mature B-cell pull is strongly reduced.
Product Categories/Family for anti-POU2F1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
76,472 Da
NCBI Official Full Name
Homo sapiens POU class 2 homeobox 1, mRNA
NCBI Official Synonym Full Names
POU class 2 homeobox 1
NCBI Official Symbol
POU2F1
NCBI Official Synonym Symbols
OCT1; OTF1; oct-1B
NCBI Protein Information
POU domain, class 2, transcription factor 1; NF-A1; OTF-1; oct-1; octamer-binding protein 1; Octamer-binding transcription factor-1; octamer-binding transcription factor 1
UniProt Protein Name
POU domain, class 2, transcription factor 1
Protein Family
UniProt Gene Name
POU2F1
UniProt Synonym Gene Names
OCT1; OTF1; Oct-1; OTF-1
UniProt Entry Name
PO2F1_HUMAN
Uniprot Description
Oct1: a ubiquitous transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR. Phosphorylation apparently inhibits its interaction with DNA. Four splice variant isoforms have been described. Isoform 2 is lymphocyte specific.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 1q24.2
Cellular Component: nucleoplasm; transcription factor complex; intracellular membrane-bound organelle; nucleus
Molecular Function: protein binding; sequence-specific DNA binding; transcription factor activity
Biological Process: transcription from RNA polymerase III promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; olfactory placode formation
Similar Products
Product Notes
The POU2F1 pou2f1 (Catalog #AAA6133066) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The POU2F1 (POU Domain, Class 2, Transcription Factor 1, NF-A1, Octamer-binding Protein 1, Oct-1, Octamer-binding Transcription Factor 1, OTF-1, OCT1, OTF1) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's POU2F1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the POU2F1 pou2f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POU2F1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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