Western Blot (WB)
(Biotinylated (0.1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Goat SNAP25 Polyclonal Antibody | anti-SNAP25 antibody
Goat Anti-SNAP25, Biotinylated Antibody
Expected from sequence similarity: Human, Mouse, Rat, Dog
Expected from sequence similarity: Human, Mouse, Rat, Dog
Peptide ELISA: Antibody detection limit dilution 1:64000.
Western Blot (WB)
(Biotinylated (0.1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Western Blot (WB)
(Biotinylated (0.1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
NCBI and Uniprot Product Information
NCBI Description
Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
SNAP-25: a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Part of the SNARE core complex containing SNAP25, VAMP2 and syntaxin 1A. Associates with proteins involved in vesicle docking and membrane fusion. Two alternatively spliced isoforms have been described.
Protein type: Vesicle
Chromosomal Location of Human Ortholog: 20p12-p11.2
Cellular Component: cytoplasm; cytosol; growth cone; membrane; neuron projection; plasma membrane; presynaptic membrane; SNARE complex; vesicle; voltage-gated potassium channel complex
Molecular Function: calcium-dependent protein binding; protein binding; SNAP receptor activity; syntaxin-1 binding
Biological Process: glutamate secretion; neurotransmitter secretion; regulation of insulin secretion; synaptic vesicle exocytosis; synaptic vesicle fusion to presynaptic membrane; synaptic vesicle priming
Disease: Myasthenic Syndrome, Congenital, 18
Research Articles on SNAP25
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Product Notes
The SNAP25 snap25 (Catalog #AAA423684) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-SNAP25, Biotinylated Antibody reacts with Tested: Human, Mouse, Rat Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's SNAP25 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Western Blot: Approx 26kDa band observed in Rat Brain lysates (calculated MW of 23.3kDa according to NP_003072.2 and NP_570824.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3ug/ml. Peptide ELISA: Antibody detection limit dilution 1:64000. Researchers should empirically determine the suitability of the SNAP25 snap25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DEANQRATKM LGSG. It is sometimes possible for the material contained within the vial of "SNAP25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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