anti-Human OCT-1 Polyclonal Antibody | anti-OCT-1 antibody

Anti-Oct-1 Antibody

Cat. Num. AAA178270

• Gene Names: POU2F1; OCT1; OTF1; oct-1B
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: OCT-1; Polyclonal Antibody; Anti-Oct-1 Antibody; POU domain; class 2; transcription factor 1; FLJ42836; NF A1; NF-A1; Oct 1; Oct 1B; Oct-1; OCT1; Octamer binding protein 1; Octamer binding transcription factor 1; Octamer-binding protein 1; Octamer-binding transcription factor 1; OTF 1; OTF-1; OTF1; OTTHUMP00000032348; OTTHUMP00000032350; OTTHUMP00000032351; PO21; PO2F1; PO2F1_HUMAN; POU class 2 homeobox 1; POU domain class 2 transcription factor 1; POU2F1; anti-OCT-1 antibody

• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 755

• Applicable Applications for anti-OCT-1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human Oct-1 recombinant protein (Position: S11-Q240). Human Oct-1 shares 96% and 99% amino acid (aa) sequence identity with mouse and rat Oct-1, respectively.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- Oct-1 Picoband antibody, MBS178270, Western blottingAll lanes: Anti Oct-1 (MBS178270) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Human Placenta Tissue Lysate at 50ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugLane 5: A549 Whole Cell Lysate at 40ugLane 6: SMMC Whole Cell Lysate at 40ugPredicted bind size: 90KDObserved bind size: 90KD )

Immunohistochemistry (IHC)

(Anti- Oct-1 Picoband antibody, MBS178270, IHC(P)IHC(P): Human Tonsil Tissue)

Related Product Information for anti-OCT-1 antibody

Description: Rabbit IgG polyclonal antibody for POU domain, class 2, transcription factor 1(POU2F1) detection. Tested with WB, IHC-P in Human.

Background: POU domain, class 2, transcription factor 1, also known as OTF1 or OCT1, is a protein that in humans is encoded by the POU2F1 gene. The POU2F1 transcription factor was among the first identified members of the POU transcription factor family. This gene is mapped to 1q24.1 to q24.2. It has been found that the POU dimer from the POU2F1 gene formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1, whereas POU dimers formed on the consensus MORE (more PORE) (ATGCATATGCAT) or on MOREs from immunoglobulin heavy chain promoters (AT[G/A][C/A]ATATGCAA) failed to interact. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA.

References

1. Hsieh, C.-L., Sturm, R., Herr, W., Francke, U. The gene for the ubiquitous octamer-binding protein Oct-1 is on human chromosome 1, region cen-q32, and near Ly-22 and Ltw-4 on mouse chromosome 1. Genomics 6: 666-672, 1990. 2. Tomilin, A., Remenyi, A., Lins, K., Bak, H., Leidel, S., Vriend, G., Wilmanns, M., Scholer, H. R.Synergism with the coactivator OBF-1 (OCA-B, BOB-1) is mediated by a specific POU dimer configuration. Cell 103: 853-864, 2000. 3. Roberts S, Segil N, Heintz N (October 1991). "Differential phosphorylation of the transcription factor Oct1 during the cell cycle". Science 253 (5023): 1022-6.

NCBI and Uniprot Product Information

• NCBI GI #: 311771667
• NCBI GeneID: 5451
• NCBI Accession #: NP_001185712.1
• NCBI GenBank Nucleotide #: NM_001198783.1
• UniProt Accession #: P14859; Q5TBT7; Q6PK46; Q8NEU9; Q9BPV1; B1AL91; B1AL93; B4E029; J3KP77
• Molecular Weight: 72,082 Da
• NCBI Official Full Name: POU domain, class 2, transcription factor 1 isoform 2
• NCBI Official Synonym Full Names: POU class 2 homeobox 1
• NCBI Official Symbol: POU2F1
• NCBI Official Synonym Symbols: OCT1; OTF1; oct-1B
• NCBI Protein Information: POU domain, class 2, transcription factor 1
• UniProt Protein Name: POU domain, class 2, transcription factor 1
• UniProt Gene Name: POU2F1
• UniProt Synonym Gene Names: OCT1; OTF1; Oct-1; OTF-1
• UniProt Entry Name: PO2F1_HUMAN

NCBI Description

The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM, Jul 2010]

Uniprot Description

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.