Rabbit anti-Mouse NXN Polyclonal Antibody | anti-NXN antibody

NXN, ID (NXN, NRX, Nucleoredoxin) (FITC)

Cat. Num. AAA6327373

• Gene Names: NXN; NRX; TRG-4; FLJ12614
• Reactivity: Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NXN; Polyclonal Antibody; ID (NXN; NRX; Nucleoredoxin) (FITC); Nucleoredoxin; anti-NXN antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-NXN antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NXN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-249 amino acids from the Central region of human NXN.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NXN Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the NXN antibody detected the NXN protein (arrow).)

Immunohistochemistry (IHC)

(NXN Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NXN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-NXN antibody

NXN functions as a redox-dependent negative regulator of the Wnt signaling pathway. May function as a transcriptional regulator. May regulate phosphatase 2A (By similarity).

Product Categories/Family for anti-NXN antibody

Antibodies; Wnt Proteins

References

Ollila, H.M., et al. Mol. Psychiatry 14(4):351-353(2009). Funato, Y., et al. Antioxid. Redox Signal. 9(8):1035-1057(2007). Hirota, K., et al. Biochem. Biophys. Res. Commun. 274(1):177-182(2000). Kurooka, H., et al. Genomics 39(3):331-339(1997).

NCBI and Uniprot Product Information

• NCBI GI #: 33149331
• NCBI GeneID: 64359
• NCBI Accession #: NP_071908.2
• NCBI GenBank Nucleotide #: NM_022463.4
• UniProt Accession #: Q6DKJ4; Q3SWW6; Q6P3U6; Q9H9Q1; D3DTH2; Q56VW5; Q7L4C6
• Molecular Weight: 48,392 Da
• NCBI Official Full Name: nucleoredoxin isoform 1
• NCBI Official Synonym Full Names: nucleoredoxin
• NCBI Official Symbol: NXN
• NCBI Official Synonym Symbols: NRX; TRG-4; FLJ12614
• NCBI Protein Information: nucleoredoxin; nucleoredoxin 1; OTTHUMP00000115356
• UniProt Protein Name: Nucleoredoxin
• Protein Family: Nucleoredoxin
• UniProt Gene Name: NXN
• UniProt Synonym Gene Names: NRX

Uniprot Description

Functions as a redox-dependent negative regulator of the Wnt signaling pathway, possibly by preventing ubiquitination of DVL3 by the BCR(KLHL12) complex. May also function as a transcriptional regulator act as a regulator of protein phosphatase 2A (PP2A) ().