Rabbit anti-Human, Mouse NUP85 Polyclonal Antibody | anti-NUP85 antibody

NUP85 Rabbit Polyclonal

Cat. Num. AAA7602162

• Gene Names: NUP85; Nup75; FROUNT
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: NUP85; Polyclonal Antibody; NUP85 Rabbit Polyclonal; anti-NUP85 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 610

• Applicable Applications for anti-NUP85 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Immunogen: Nucleoporin 85kDa
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach using MBS7602162 (NUP85 antibody) at dilution of 1:50)

Western Blot (WB)

(mouse lung tissue were subjected to SDS PAGE followed by western blot with MBS7602162 (NUP85 antibody) at dilution of 1:400)

NCBI and Uniprot Product Information

• NCBI GI #: 740086933
• NCBI GeneID: 79902
• NCBI Accession #: NP_001290205.1
• NCBI GenBank Nucleotide #: NM_001303276.1
• Molecular Weight: 69,800 Da
• NCBI Official Full Name: nuclear pore complex protein Nup85 isoform 2
• NCBI Official Synonym Full Names: nucleoporin 85
• NCBI Official Symbol: NUP85
• NCBI Official Synonym Symbols: Nup75; FROUNT
• NCBI Protein Information: nuclear pore complex protein Nup85
• UniProt Protein Name: Nuclear pore complex protein Nup85
• Protein Family: Nucleoporin
• UniProt Gene Name: NUP85
• UniProt Synonym Gene Names: NUP75; PCNT1
• UniProt Entry Name: NUP85_HUMAN

NCBI Description

This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. Nuclear pore complexes are embedded in the nuclear envelope and promote bidirectional transport of macromolecules between the cytoplasm and nucleus. The encoded protein can also bind to the C-terminus of chemokine (C-C motif) receptor 2 (CCR2) and promote chemotaxis of monocytes, thereby participating in the inflammatory response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

NUP85: Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP98/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2- activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl- inositol 3-kinase-Rac-lammellipodium protrusion cascade. Belongs to the nucleoporin Nup85 family.

Protein type: Nucleoporin

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: cytosol; kinetochore; membrane; nuclear envelope; nuclear membrane; spindle

Molecular Function: protein binding; structural constituent of nuclear pore

Biological Process: lamellipodium biogenesis; macrophage chemotaxis; mitotic nuclear envelope disassembly; mRNA export from nucleus; positive regulation of transcription, DNA-dependent; protein import into nucleus; protein sumoylation; RNA-mediated gene silencing; sister chromatid cohesion; tRNA export from nucleus; viral reproduction; viral transcription