Mouse anti-Human AKAP12 Monoclonal Antibody | anti-AKAP12 antibody

AKAP12 (A-kinase Anchor Protein 12, AKAP-12, A-kinase Anchor Protein 250kD, AKAP 250, Gravin, Myasthenia Gravis Autoantigen, AKAP250, DKFZp686M0430, DKFZp686O0331, FLJ20945, FLJ97621) (PE)

Cat. Num. AAA6156444

• Gene Names: AKAP12; SSeCKS; AKAP250
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AKAP12; Monoclonal Antibody; AKAP12 (A-kinase Anchor Protein 12; AKAP-12; A-kinase Anchor Protein 250kD; AKAP 250; Gravin; Myasthenia Gravis Autoantigen; AKAP250; DKFZp686M0430; DKFZp686O0331; FLJ20945; FLJ97621) (PE); anti-AKAP12 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 1C5
• Specificity: Recognizes human AKAP12.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-AKAP12 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa1675-1782 from AKAP12 (NP_005091) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: VPEDDGHALLAERIEKSLVEPKEDEKGDDVDDPENQNSALADTDASGGLTKESPDTNGPKQKEKEDAQEVELQEGKVHSESDKAITPQAQEELQKQERESAKSELTES
• Conjugate: PE
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (37.62kD).)

Testing Data

(Detection limit for recombinant GST tagged AKAP12 is ~0.03ng/ml as a capture antibody.)

Related Product Information for anti-AKAP12 antibody

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternate splicing of this gene results in many isoforms that localize to the centrosome and the Golgi apparatus, and interact with numerous signaling proteins from multiple signal transduction pathways. These signaling proteins include type II protein kinase A, serine/threonine kinase protein kinase N, protein phosphatase 1, protein phosphatase 2a, protein kinase C-epsilon and phosphodiesterase 4D3.

Product Categories/Family for anti-AKAP12 antibody

Antibodies; Protein Kinases

References

1. Quantitative and temporal proteome analysis of butyrate-treated colorectal cancer cells. Tan HT, Tan S, Lin Q, Lim TK, Hew CL, Chung MC.Mol Cell Proteomics. 2008 Jun;7(6):1174-85. Epub 2008 Mar 14.

NCBI and Uniprot Product Information

• NCBI GI #: 21493022
• NCBI GeneID: 9590
• NCBI Accession #: NP_005091
• NCBI GenBank Nucleotide #: NM_005100
• UniProt Accession #: Q86TJ9; Q02952
• Molecular Weight: 35,345 Da
• NCBI Official Full Name: A-kinase anchor protein 12 isoform 1
• NCBI Official Synonym Full Names: A kinase (PRKA) anchor protein 12
• NCBI Official Symbol: AKAP12
• NCBI Official Synonym Symbols: SSeCKS; AKAP250
• NCBI Protein Information: A-kinase anchor protein 12; A-kinase anchor protein, 250kDa; AKAP 250; Src-Suppressed C Kinase Substrate; kinase scaffold protein gravin; myasthenia gravis autoantigen gravin
• UniProt Protein Name: AKAP12 protein
• Protein Family: A-kinase anchor protein
• UniProt Gene Name: AKAP12
• UniProt Entry Name: Q86TJ9_HUMAN