Rabbit anti-Human Guanylate Cyclase beta-1, Soluble Polyclonal Antibody | anti-GCS-beta-1 antibody

Guanylate Cyclase beta-1, Soluble (GCS-beta-1, GC-S-beta-1, GUCY1B1, Guanylate Cyclase 1 Soluble beta 3, GUC1B3, GUCY1B3, Guanylate Cyclase Soluble Subunit beta-3, GCS-beta-3, GC-SB3, GUCB3, GUCSB3, Soluble Guanylate Cyclase Small Subunit) (Biotin)

Cat. Num. AAA6479333

• Gene Names: GUCY1B1; GUCB3; GC-SB3; GUC1B3; GUCSB3; GUCY1B3; GC-S-beta-1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Guanylate Cyclase beta-1; Soluble; Polyclonal Antibody; Soluble (GCS-beta-1; GC-S-beta-1; GUCY1B1; Guanylate Cyclase 1 Soluble beta 3; GUC1B3; GUCY1B3; Guanylate Cyclase Soluble Subunit beta-3; GCS-beta-3; GC-SB3; GUCB3; GUCSB3; Soluble Guanylate Cyclase Small Subunit) (Biotin); EC=4.6.1.2; Guanylate Cyclase Soluble Subunit beta-1; anti-GCS-beta-1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GUCY1B3.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-GCS-beta-1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human GUCY1B3 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-GUCY1B3 Pab(AP8171b) in Y79 cell line lysate. GUCY1B3(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-GCS-beta-1 antibody

Antibodies; Enzymes; Cyclase

References

1. Russwurm, M., et al., J. Biol. Chem. 276(48):44647-44652 (2001). 2. Lucas, K.A., et al., Pharmacol. Rev. 52(3):375-414 (2000). 3. Papapetropoulos, A., et al., J. Cell. Physiol. 167(2):213-221 (1996). 4. Giuili, G., et al., Hum. Genet. 91(3):257-260 (1993). 5. Giuili, G., et al., FEBS Lett. 304(1):83-88 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 4504215
• NCBI GeneID: 2983
• NCBI Accession #: NP_000848
• NCBI GenBank Nucleotide #: NM_000857
• UniProt Accession #: Q02153
• Molecular Weight: 71 kDa
• NCBI Official Full Name: guanylate cyclase soluble subunit beta-1 isoform 2
• NCBI Official Synonym Full Names: guanylate cyclase 1 soluble subunit beta 1
• NCBI Official Symbol: GUCY1B1
• NCBI Official Synonym Symbols: GUCB3; GC-SB3; GUC1B3; GUCSB3; GUCY1B3; GC-S-beta-1
• NCBI Protein Information: guanylate cyclase soluble subunit beta-1
• UniProt Protein Name: Guanylate cyclase soluble subunit beta-1
• UniProt Gene Name: GUCY1B3
• UniProt Synonym Gene Names: GUC1B3; GUCSB3; GUCY1B1; GCS-beta-1; GCS-beta-3
• UniProt Entry Name: GCYB1_HUMAN

NCBI Description

This gene encodes the beta subunit of the soluble guanylate cyclase (sGC), which catalyzes the conversion of GTP (guanosine triphosphate) to cGMP (cyclic guanosine monophosphate). The encoded protein contains an HNOX domain, which serves as a receptor for ligands such as nitric oxide, oxygen and nitrovasodilator drugs. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

GUCY1B3: Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Heterodimer of an alpha and a beta chain. 2 isoforms of the human protein are produced by alternative splicing

Protein type: EC 4.6.1.2; Guanylyl cyclase; Nucleotide Metabolism - purine; Receptor, misc.; Lyase

Chromosomal Location of Human Ortholog: 4q31.3-q33

Cellular Component: guanylate cyclase complex, soluble; intracellular membrane-bound organelle; cytoplasm

Molecular Function: guanylate cyclase activity; GTP binding; protein heterodimerization activity; metal ion binding; heme binding; Hsp90 protein binding; receptor activity

Biological Process: cGMP biosynthetic process; blood circulation; blood coagulation; nitric oxide mediated signal transduction