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Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells, using Progesterone Receptor (Phospho-Ser294) Antibody. The picture on the right is blocked with the phospho peptide.)

Rabbit Progesterone Receptor Polyclonal Antibody | anti-PGR antibody

Progesterone Receptor (Phospho-Ser294) Antibody

Gene Names
PGR; PR; NR3C3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Synonyms
Progesterone Receptor; Polyclonal Antibody; Progesterone Receptor (Phospho-Ser294) Antibody; anti-PGR antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Progesterone Receptor (Phospho-Ser294) Antibody detects endogenous levels of Progesterone Receptor only when phosphorylated at Ser294.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
831
Applicable Applications for anti-PGR antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:1000
Target Modification
Phospho
Modification Sites
H:S294; M:S294; R:S293
Immunogen
The antiserum was produced against synthesized peptide derived from human Progesterone Receptor around the phosphorylation site of Ser294.
Immunogen Range
261-310
Preparation and Storage
Stable at -20 degree C for at least 1 year.

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells, using Progesterone Receptor (Phospho-Ser294) Antibody. The picture on the right is blocked with the phospho peptide.)

Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells, using Progesterone Receptor (Phospho-Ser294) Antibody. The picture on the right is blocked with the phospho peptide.)

Western Blot (WB)

(Western blot analysis of lysates from 293 cells treated with Etoposide 25uM 60', using Progesterone Receptor (Phospho-Ser294) Antibody. The lane on the right is blocked with the phospho peptide.)

Western Blot (WB) (Western blot analysis of lysates from 293 cells treated with Etoposide 25uM 60', using Progesterone Receptor (Phospho-Ser294) Antibody. The lane on the right is blocked with the phospho peptide.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
98 kDa
NCBI Official Full Name
progesterone receptor isoform B
NCBI Official Synonym Full Names
progesterone receptor
NCBI Official Symbol
PGR
NCBI Official Synonym Symbols
PR; NR3C3
NCBI Protein Information
progesterone receptor
UniProt Protein Name
Progesterone receptor
Protein Family
UniProt Gene Name
PGR
UniProt Synonym Gene Names
NR3C3; PR

NCBI Description

This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce several transcript variants, both protein coding and non-protein coding. Two of the isoforms (A and B) are identical except for an additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. [provided by RefSeq, Sep 2015]

Uniprot Description

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Depending on the isoform, progesterone receptor functions as transcriptional activator or repressor.

Research Articles on PGR

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Product Notes

The PGR pgr (Catalog #AAA9502533) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Progesterone Receptor (Phospho-Ser294) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Progesterone Receptor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000. Researchers should empirically determine the suitability of the PGR pgr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Progesterone Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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