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Western Blot (WB) (Western blot detection of TREX1 in Hela cell lysates using TREX1 Rabbit mAb(1:1000 diluted). Predicted band size:39kDa. Observed band size:33kDa.)

Rabbit anti-Human TREX1 Monoclonal Antibody | anti-TREX1 antibody

TREX1 Rabbit mAb

Gene Names
TREX1; CRV; AGS1; DRN3; HERNS
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Affinity purified
Synonyms
TREX1; Monoclonal Antibody; TREX1 Rabbit mAb; CRV; AGS1; DRN3; HERNS; anti-TREX1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
R05-1D6
Specificity
Detected in thymus, spleen, liver, brain, heart, small intestine and colon.
Purity/Purification
Affinity purified
Form/Format
Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applicable Applications for anti-TREX1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:1000-1:5000
IHC: 1:20-1:50
ICC/IF: 1:50
Immunogen
Recombinant protein of human TREX1
Conjugation
Unconjugated
Modification
Unmodification
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot detection of TREX1 in Hela cell lysates using TREX1 Rabbit mAb(1:1000 diluted). Predicted band size:39kDa. Observed band size:33kDa.)

Western Blot (WB) (Western blot detection of TREX1 in Hela cell lysates using TREX1 Rabbit mAb(1:1000 diluted). Predicted band size:39kDa. Observed band size:33kDa.)
Related Product Information for anti-TREX1 antibody
Major cellular 3'-to-5' DNA exonuclease which digests single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) with mismatched 3' termini. Prevents cell-intrinsic initiation of autoimmunity. Acts by metabolizing DNA fragments from endogenous retroelements, including L1, LTR and SINE elements. Unless degraded, these DNA fragments accumulate in the cytosol and activate the IFN-stimulatory DNA (ISD) response and innate immune signaling. Prevents chronic ATM-dependent checkpoint activation, by processing ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates. Inefficiently degrades oxidized DNA, such as that generated upon antimicrobial reactive oxygen production or upon absorption of UV light. During GZMA-mediated cell death, contributes to DNA damage in concert with NME1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,212 Da
NCBI Official Full Name
three-prime repair exonuclease 1 isoform a
NCBI Official Synonym Full Names
three prime repair exonuclease 1
NCBI Official Symbol
TREX1
NCBI Official Synonym Symbols
CRV; AGS1; DRN3; HERNS
NCBI Protein Information
three-prime repair exonuclease 1; DNase III; deoxyribonuclease III; 3' repair exonuclease 1; 3'-5' exonuclease TREX1
UniProt Protein Name
Three-prime repair exonuclease 1
UniProt Gene Name
TREX1
UniProt Entry Name
TREX1_HUMAN

NCBI Description

This gene encodes a nuclear protein with 3' exonuclease activity. The encoded protein may play a role in DNA repair and serve as a proofreading function for DNA polymerase. Mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, Cree encephalitis, and other diseases of the immune system. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012]

Uniprot Description

TREX1: the major 3' DNA exonuclease in mammalian cells. Normally associates with the endoplasmic reticulum (ER). Translocates to the nucleus at S phase after DNA damage by gamma-irradiation or hydroxyurea. Trex1-deficient cells show defective G1/S transition, with single-stranded DNA molecules persisting after S phase and accumulating in the ER. Degrades ssDNA. Prevents chronic checkpoint activation and inappropriate immune activation. Mutations cause Aicardi-Goutieres syndrome, an autoimmune disorder. Trex1a(-/-) mice have autoinflammatory responses. The gene for this protein is either identical to or adjacent to that of ATRIP. Some of the mRNAs that encode TREX1 also encode ATRIP in another reading frame. Three alternatively spliced human isoforms have been reported.

Protein type: EC 3.1.11.2; Cell cycle regulation; DNA-binding; DNA repair, damage; Deoxyribonuclease

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: endoplasmic reticulum membrane; nucleolus; nuclear envelope; nucleus; cytosol

Molecular Function: protein homodimerization activity; metal ion binding; double-stranded DNA binding; exodeoxyribonuclease III activity; 3'-5'-exodeoxyribonuclease activity; MutLalpha complex binding; adenyl deoxyribonucleotide binding; 3'-5' exonuclease activity; MutSalpha complex binding; single-stranded DNA binding

Biological Process: regulation of interferon type I production; mismatch repair; positive regulation of interferon type I production; innate immune response; DNA replication; DNA repair; DNA catabolic process, exonucleolytic; DNA metabolic process; DNA recombination

Disease: Chilblain Lupus 1; Vasculopathy, Retinal, With Cerebral Leukodystrophy; Aicardi-goutieres Syndrome 1; Systemic Lupus Erythematosus

Research Articles on TREX1

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Product Notes

The TREX1 trex1 (Catalog #AAA4752434) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TREX1 Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TREX1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:1000-1:5000 IHC: 1:20-1:50 ICC/IF: 1:50. Researchers should empirically determine the suitability of the TREX1 trex1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TREX1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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