Rabbit anti-Human, Mouse NUDT4 Polyclonal Antibody | anti-NUDT4 antibody

NUDT4, NT (NUDT4, DIPP2, KIAA0487, Diphosphoinositol polyphosphate phosphohydrolase 2, Diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 2, Nucleoside diphosphate-linked moiety X motif 4) (APC)

Cat. Num. AAA6327162

• Gene Names: NUDT4; DIPP2; NUDT4B; DIPP-2B; HDCMB47P; DIPP2beta; DIPP2alpha
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NUDT4; Polyclonal Antibody; NT (NUDT4; DIPP2; KIAA0487; Diphosphoinositol polyphosphate phosphohydrolase 2; Diadenosine 5'; 5'''-P1; P6-hexaphosphate hydrolase 2; Nucleoside diphosphate-linked moiety X motif 4) (APC); Nucleoside diphosphate-linked moiety X motif 4; anti-NUDT4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 181

• Applicable Applications for anti-NUDT4 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NUDT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human NUDT4.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NUDT4 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the NUDT4 antibody detected the NUDT4 protein (arrow).)

Western Blot (WB)

(NUDT4 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the NUDT4 antibody detected the NUDT4 protein (arrow).)

Related Product Information for anti-NUDT4 antibody

The protein encoded by this gene regulates the turnover of diphosphoinositol polyphosphates. The turnover of these high-energy diphosphoinositol polyphosphates represents a molecular switching activity with important regulatory consequences. Molecular switching by diphosphoinositol polyphosphates may contribute to regulating intracellular trafficking. Several alternatively spliced transcript variants have been described, but the full-length nature of some variants has not been determined. Isoforms DIPP2alpha and DIPP2beta are distinguishable from each other solely by DIPP2beta possessing one additional amino acid due to intron boundary skidding in alternate splicing.

Product Categories/Family for anti-NUDT4 antibody

Antibodies; KIAA; orf

References

Fortna, A., et al. PLoS Biol. 2 (7), E207 (2004): Fisher, D.I., et al. J. Biol. Chem. 277(49):47313-47317(2002). Leslie, N.R., et al. BMC Biochem. 3, 20 (2002): Caffrey, J.J., et al. Gene 269 (1-2), 53-60 (2001): Caffrey, J.J., et al. J. Biol. Chem. 275(17):12730-12736(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 40317634
• NCBI GeneID: 11163
• NCBI Accession #: NP_950241.1
• NCBI GenBank Nucleotide #: NP_950241.1
• UniProt Accession #: Q9NZJ9
• NCBI Official Full Name: diphosphoinositol polyphosphate phosphohydrolase 2 isoform beta
• NCBI Official Synonym Full Names: nudix hydrolase 4
• NCBI Official Symbol: NUDT4
• NCBI Official Synonym Symbols: DIPP2; NUDT4B; DIPP-2B; HDCMB47P; DIPP2beta; DIPP2alpha
• NCBI Protein Information: diphosphoinositol polyphosphate phosphohydrolase 2
• UniProt Protein Name: Diphosphoinositol polyphosphate phosphohydrolase 2
• Protein Family: Nudix hydrolase
• UniProt Gene Name: NUDT4
• UniProt Synonym Gene Names: DIPP2; KIAA0487; DIPP-2; Nudix motif 4
• UniProt Entry Name: NUDT4_HUMAN

NCBI Description

The protein encoded by this gene regulates the turnover of diphosphoinositol polyphosphates. The turnover of these high-energy diphosphoinositol polyphosphates represents a molecular switching activity with important regulatory consequences. Molecular switching by diphosphoinositol polyphosphates may contribute to regulating intracellular trafficking. Several alternatively spliced transcript variants have been described, but the full-length nature of some variants has not been determined. Isoforms DIPP2alpha and DIPP2beta are distinguishable from each other solely by DIPP2beta possessing one additional amino acid due to intron boundary skidding in alternate splicing. [provided by RefSeq, Jul 2008]

Uniprot Description

NUDT4: Cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate), PP-InsP4 and [PP]2-InsP4 (bisdiphosphoinositol tetrakisphosphate), suggesting that it may play a role in signal transduction. Also able to catalyze the hydrolysis of dinucleoside oligophosphate Ap6A, but not Ap5A. The major reaction products are ADP and p4a from Ap6A. Also able to hydrolyze 5-phosphoribose 1-diphosphate. Belongs to the Nudix hydrolase family. DIPP subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.1.52; Hydrolase

Chromosomal Location of Human Ortholog: 12q21

Cellular Component: intracellular; cytosol

Molecular Function: snoRNA binding; m7G(5')pppN diphosphatase activity; metal ion binding; diphosphoinositol-polyphosphate diphosphatase activity

Biological Process: cyclic-nucleotide-mediated signaling; inositol phosphate metabolic process; cyclic nucleotide metabolic process; intracellular transport; calcium-mediated signaling; regulation of RNA export from nucleus