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Dot Blot (DB) (Dot blot analysis of anti-APG3-cleaved Pab on nitrocellulose membrane. 50ng of Cleaved-peptide or Non Cleaved-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

Rabbit anti-Human APG3 Polyclonal Antibody | anti-APG3 antibody

APG3, cleaved, Phosphorylated (ATG3, APG3, APG3L, Ubiquitin-like-conjugating enzyme ATG3, Autophagy-related protein 3, Protein PC3-96) (PE)

Gene Names
ATG3; APG3; APG3L; PC3-96; APG3-LIKE
Reactivity
Human
Applications
Dot Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
APG3; Polyclonal Antibody; cleaved; Phosphorylated (ATG3; APG3L; Ubiquitin-like-conjugating enzyme ATG3; Autophagy-related protein 3; Protein PC3-96) (PE); ATG3; Protein PC3-96; anti-APG3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-APG3 antibody
FLISA, Dot Blot (DB)
Application Notes
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
APG3-cleaved Antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from human APG3.
Conjugate
PE
Antigen Modification
Phosphorylated
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-APG3-cleaved Pab on nitrocellulose membrane. 50ng of Cleaved-peptide or Non Cleaved-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

Dot Blot (DB) (Dot blot analysis of anti-APG3-cleaved Pab on nitrocellulose membrane. 50ng of Cleaved-peptide or Non Cleaved-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Product Categories/Family for anti-APG3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35,467 Da
NCBI Official Full Name
ubiquitin-like-conjugating enzyme ATG3 isoform 1
NCBI Official Synonym Full Names
autophagy related 3
NCBI Official Symbol
ATG3
NCBI Official Synonym Symbols
APG3; APG3L; PC3-96; APG3-LIKE
NCBI Protein Information
ubiquitin-like-conjugating enzyme ATG3; hApg3; 2610016C12Rik; autophagy-related protein 3; ATG3 autophagy related 3 homolog
UniProt Protein Name
Ubiquitin-like-conjugating enzyme ATG3
UniProt Gene Name
ATG3
UniProt Synonym Gene Names
APG3; APG3L; APG3-like; hApg3
UniProt Entry Name
ATG3_HUMAN

NCBI Description

This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

ATG3: an E2-like enzyme essential for Apg8p lipidation.

Protein type: Enzyme, cellular metabolism; EC 6.3.2.-; Autophagy; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3q13.2

Cellular Component: cytoplasmic ubiquitin ligase complex; cytosol

Molecular Function: protein binding; enzyme binding; small conjugating protein ligase activity; APG8 conjugating enzyme activity; APG12 conjugating enzyme activity

Biological Process: mitochondrion degradation; protein ubiquitination; protein modification process; mitochondrial fragmentation during apoptosis; protein targeting to membrane; autophagic vacuole formation

Research Articles on APG3

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Product Notes

The APG3 atg3 (Catalog #AAA6274235) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The APG3, cleaved, Phosphorylated (ATG3, APG3, APG3L, Ubiquitin-like-conjugating enzyme ATG3, Autophagy-related protein 3, Protein PC3-96) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's APG3 can be used in a range of immunoassay formats including, but not limited to, FLISA, Dot Blot (DB). FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the APG3 atg3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APG3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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