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NuDT4 recombinant protein

NuDT4, 1-180aa, Human, His tag, E Coli

Gene Names
NUDT4; DIPP2; HDCMB47P; DIPP2beta; DIPP2alpha
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
NuDT4; 1-180aa; Human; His tag; E Coli; Diphosphoinositol polyphosphate phosphohydrolase 2; DIPP2; DIPP2alpha; DIPP2beta; HDCMB47P; NuDT4 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol 0.1M NaCl
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMMKFKPN QTRTYDREGF KKRAACLCFR SEQEDEVLLV SSSRYPDQWI VPGGGMEPEE EPGGAAVREV YEEAGVKGKL GRLLGIFENQ DRKHRTYVYV LTVTEILEDW EDSVNIGRKR EWFKVEDAIK VLQCHKPVHA EYLEKLKLGC SPANGNSTVP SLPDNNALFV TAAQTSGLPS SVR
Sequence Length
180
Applicable Applications for NuDT4 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for NuDT4 recombinant protein
NuDT4 regulates the turnover of diphosphoinositol polyphosphates. The turnover of these high-energy diphosphoinositol polyphosphates represents a molecular switching activity with important regulatory consequences. Molecular switching by diphosphoinositol polyphosphates may contribute to regulating intracellular trafficking. Recombinant human NuDT4 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.
Product Categories/Family for NuDT4 recombinant protein
References
Caffrey JJ. et al. (2000) J Biol Chem. 275:12730-12736; Caffrey JJ. et al. (2001) Gene. 269 :53-60.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22.7kDa (203aa) confirmed by MALDI-TOF
NCBI Official Full Name
diphosphoinositol polyphosphate phosphohydrolase 2 isoform alpha
NCBI Official Synonym Full Names
nudix hydrolase 4
NCBI Official Symbol
NUDT4
NCBI Official Synonym Symbols
DIPP2; HDCMB47P; DIPP2beta; DIPP2alpha
NCBI Protein Information
diphosphoinositol polyphosphate phosphohydrolase 2
UniProt Protein Name
Diphosphoinositol polyphosphate phosphohydrolase 2
Protein Family
UniProt Gene Name
NUDT4
UniProt Synonym Gene Names
DIPP2; KIAA0487; DIPP-2; Nudix motif 4

NCBI Description

The protein encoded by this gene regulates the turnover of diphosphoinositol polyphosphates. The turnover of these high-energy diphosphoinositol polyphosphates represents a molecular switching activity with important regulatory consequences. Molecular switching by diphosphoinositol polyphosphates may contribute to regulating intracellular trafficking. Several alternatively spliced transcript variants have been described, but the full-length nature of some variants has not been determined. Isoforms DIPP2alpha and DIPP2beta are distinguishable from each other solely by DIPP2beta possessing one additional amino acid due to intron boundary skidding in alternate splicing. [provided by RefSeq, Jul 2008]

Uniprot Description

Cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate), PP-InsP4 and [PP]2-InsP4 (bisdiphosphoinositol tetrakisphosphate), suggesting that it may play a role in signal transduction. Also able to catalyze the hydrolysis of dinucleoside oligophosphate Ap6A, but not Ap5A. The major reaction products are ADP and p4a from Ap6A. Also able to hydrolyze 5-phosphoribose 1-diphosphate. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA.

Research Articles on NuDT4

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Product Notes

The NuDT4 nudt4 (Catalog #AAA204958) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's NuDT4 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the NuDT4 nudt4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMMKFKPN QTRTYDREGF KKRAACLCFR SEQEDEVLLV SSSRYPDQWI VPGGGMEPEE EPGGAAVREV YEEAGVKGKL GRLLGIFENQ DRKHRTYVYV LTVTEILEDW EDSVNIGRKR EWFKVEDAIK VLQCHKPVHA EYLEKLKLGC SPANGNSTVP SLPDNNALFV TAAQTSGLPS SVR. It is sometimes possible for the material contained within the vial of "NuDT4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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