Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NSFL1C antibody.)
Rabbit NSFL1C Polyclonal Antibody | anti-NSFL1C antibody
NSFL1C antibody
Immunofluorescence: 1:50 - 1:200
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NSFL1C antibody.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NSFL1C antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cell using NSFL1C antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cell using NSFL1C antibody. Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
NCBI Description
N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. [provided by RefSeq, May 2011]
Uniprot Description
NSFL1C: Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP- mediated formation of transitional endoplasmic reticulum (tER). Inhibits the activity of CTSL (in vitro). Belongs to the NSFL1C family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Vesicle
Chromosomal Location of Human Ortholog: 20p13
Cellular Component: nucleoplasm; intermediate filament cytoskeleton; Golgi stack; cytoplasm; plasma membrane; chromosome; cytosol
Molecular Function: protein binding; ubiquitin binding; protein phosphatase type 1 regulator activity; lipid binding
Biological Process: proteasomal ubiquitin-dependent protein catabolic process; regulation of catalytic activity; nuclear envelope reassembly; Golgi organization and biogenesis; autophagic vacuole formation
Research Articles on NSFL1C
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Product Notes
The NSFL1C nsfl1c (Catalog #AAA9405028) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NSFL1C antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NSFL1C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Western blotting: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200. Researchers should empirically determine the suitability of the NSFL1C nsfl1c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NSFL1C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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