Western Blot (WB)
(Western Blot (WB) analysis of NSFL1C polyclonal antibody)
Rabbit NSFL1C Polyclonal Antibody | anti-NSFL1C antibody
NSFL1C Polyclonal Antibody
Gene Names
NSFL1C; P47; UBX1; UBXD10; UBXN2C; dJ776F14.1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunoprecipitation
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
NSFL1C; Polyclonal Antibody; NSFL1C Polyclonal Antibody; NSFL1 cofactor p47; UBX domain-containing protein 2C; p97 cofactor p47; UBXN2C; anti-NSFL1C antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
NSFL1C polyclonal antibody detects endogenous levels of NSFL1C protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
370
Applicable Applications for anti-NSFL1C antibody
Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IF: 1:50-1:200
IP: 1:50-1:100
IF: 1:50-1:200
IP: 1:50-1:100
Immunogen
Recombinant full length Human NSFL1C.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of NSFL1C polyclonal antibody)
Western Blot (WB)
(Western Blot (WB) analysis of NSFL1C polyclonal antibody)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug NSFL1C antibody.)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug NSFL1C antibody.)
Related Product Information for anti-NSFL1C antibody
p47, also known as NSFL1C, UBX1, UBXD10 or UBXN2C, is a 370 amino acid protein that localizes to both the nucleus and the golgi apparatus (specifically to golgi stacks) and contains one SEP domain and one UBX domain. Functioning as part of a ternary complex with VCP (a protein involved in the heterotypic fusion of transport vesicles with their target membranes) and Syntaxin 5, p47 interacts with and reduces the ATPase activity of VCP and is required for the fragmentation of golgi stacks during mitosis and for subsequent reassembly of golgi stacks after mitosis. p47 is subject to phosphorylation during mitosis, which inhibits p47-golgi interaction and is, therefore, required for proper golgi stack formation and cisternal regrowth. Human p47 shares 89% sequence identity with its mouse counterpart, suggesting a conserved role between species. Multiple isoforms of p47 exist due to alternative splicing events.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 41kDa
NCBI Official Full Name
NSFL1 cofactor p47 isoform a
NCBI Official Synonym Full Names
NSFL1 cofactor
NCBI Official Symbol
NSFL1C
NCBI Official Synonym Symbols
P47; UBX1; UBXD10; UBXN2C; dJ776F14.1
NCBI Protein Information
NSFL1 cofactor p47
UniProt Protein Name
NSFL1 cofactor p47
Protein Family
UniProt Gene Name
NSFL1C
UniProt Synonym Gene Names
UBXN2C
UniProt Entry Name
NSF1C_HUMAN
NCBI Description
N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. [provided by RefSeq, May 2011]
Research Articles on NSFL1C
Similar Products
Product Notes
The NSFL1C nsfl1c (Catalog #AAA3011664) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NSFL1C Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NSFL1C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 IF: 1:50-1:200 IP: 1:50-1:100. Researchers should empirically determine the suitability of the NSFL1C nsfl1c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NSFL1C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
