Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NSA2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
Rabbit NSA2 Polyclonal Antibody | anti-NSA2 antibody
NSA2 Polyclonal Antibody
Gene Names
NSA2; HCLG1; TINP1; CDK105; HCL-G1; HUSSY29; HUSSY-29
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity Purification
Synonyms
NSA2; Polyclonal Antibody; NSA2 Polyclonal Antibody; CDK105; HCL-G1; HCLG1; HUSSY-29; HUSSY29; TINP1; anti-NSA2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence
MPQNEYIELHRKRYGYRLDYHEKKRKKESREAHERSKKAKKMIGLKAKLYHKQRHAEKIQMKKTIKMHEKRNTKQKNDEKTPQGAVPAYLLDREGQSRAKVLSNMIKQKRKEKAGKWEVPLPKVRAQGETEVLKVIRTGKRKKKAWKRMVTKVCFVGDGFTRKPPKYERFIRPMGLRFKKAHVTHPELKATFCLPILGVKKNPSSPLYTTLGVITKGTVIEVNVSELGLVTQGGKVIWGKYAQVTNNPENDGCIN
Sequence Length
189
Applicable Applications for anti-NSA2 antibody
Western Blot (WB)
Application Notes
WB: 1:500 - 1:2000
Immunogen
Recombinant protein of human NSA2
Immunogen Species
Human
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Nucleus, nucleolus
Positive Samples
HeLa, 293T, Mouse heart, Mouse kidney, Mouse skeletal muscle, Rat heart, Rat kidney, Rat skeletal muscle
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NSA2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NSA2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
Related Product Information for anti-NSA2 antibody
This gene encodes a nucleolar protein involved in cell cycle regulation and proliferation. This gene was identified based on sequence similarity to a highly conserved Saccharomyces cerevisiae gene encoding a pre-ribosomal protein, which is involved in large ribosomal subunit biogenesis. The encoded protein is found at elevated levels in diabetic nephropathy. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified.
Product Categories/Family for anti-NSA2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated: 30kDa
Observed: 37kDa
Observed: 37kDa
NCBI Official Full Name
ribosome biogenesis protein NSA2 homolog isoform 2
NCBI Official Synonym Full Names
NSA2, ribosome biogenesis homolog
NCBI Official Symbol
NSA2
NCBI Official Synonym Symbols
HCLG1; TINP1; CDK105; HCL-G1; HUSSY29; HUSSY-29
NCBI Protein Information
ribosome biogenesis protein NSA2 homolog
UniProt Protein Name
Ribosome biogenesis protein NSA2 homolog
Protein Family
UniProt Gene Name
NSA2
UniProt Synonym Gene Names
TINP1
NCBI Description
This gene encodes a nucleolar protein involved in cell cycle regulation and proliferation. This gene was identified based on sequence similarity to a highly conserved Saccharomyces cerevisiae gene encoding a pre-ribosomal protein, which is involved in large ribosomal subunit biogenesis. The encoded protein is found at elevated levels in diabetic nephropathy. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified. [provided by RefSeq, Nov 2012]
Uniprot Description
Involved in the biogenesis of the 60S ribosomal subunit. May play a part in the quality control of pre-60S particles ().
Research Articles on NSA2
Similar Products
Product Notes
The NSA2 nsa2 (Catalog #AAA9134478) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NSA2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NSA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the NSA2 nsa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MPQNEYIELH RKRYGYRLDY HEKKRKKESR EAHERSKKAK KMIGLKAKLY HKQRHAEKIQ MKKTIKMHEK RNTKQKNDEK TPQGAVPAYL LDREGQSRAK VLSNMIKQKR KEKAGKWEVP LPKVRAQGET EVLKVIRTGK RKKKAWKRMV TKVCFVGDGF TRKPPKYERF IRPMGLRFKK AHVTHPELKA TFCLPILGVK KNPSSPLYTT LGVITKGTVI EVNVSELGLV TQGGKVIWGK YAQVTNNPEN DGCIN. It is sometimes possible for the material contained within the vial of "NSA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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