Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with CD19 antibody (eFluor 450) and Mouse IgG1 K Isotype Control (left) or CD32 antibody (right). Cells in the lymphocyte gate were used for analysis.)
Mouse CD32 Monoclonal Antibody | anti-CD32 antibody
CD32 antibody (allophycocyanin)
Applications
Flow Cytometry, Functional Assay
Synonyms
CD32; Monoclonal Antibody; CD32 antibody (allophycocyanin); Monoclonal CD32; Anti-CD32; Low Affinity IgG Receptor 2a; CD-32; CD32 (APC); FcGR2a; CD 32; IGFR2; anti-CD32 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
6C4
Specificity
Human
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Sequence Length
293
Applicable Applications for anti-CD32 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: <= 0.5 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Biological Significance
This 6C4 monoclonal antibody reacts with human CD32. This antibody recognizes two isoforms of the receptor, FcgammaRIIA and FcgammaRIIB. These 40-kDa polymorphic transmembrane glycoproteins are expressed on B cells, granulocytes, monocytes, macrophages, and platelets. Moreover, these receptors are detected on natural killer cells. CD32 enables interaction between FcgammaRII-expressing cells and opsonized antigen or IgG-containing immune complexes. Both receptors exhibit low affinity towards IgG and play a role in inflammation and autoimmune disease. This clone has been reported to inhibit Ig binding in a rosette blocking assay.
Tag/Conjugate
Allophycocyanin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with CD19 antibody (eFluor 450) and Mouse IgG1 K Isotype Control (left) or CD32 antibody (right). Cells in the lymphocyte gate were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with CD19 antibody (eFluor 450) and Mouse IgG1 K Isotype Control (left) or CD32 antibody (right). Cells in the lymphocyte gate were used for analysis.)
Related Product Information for anti-CD32 antibody
Mouse monoclonal CD32 antibody (allophycocyanin)
Product Categories/Family for anti-CD32 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The CD32 (Catalog #AAA834165) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD32 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: <= 0.5 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the CD32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD32, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
