Goat PAX8 Polyclonal Antibody | anti-PAX8 antibody

Goat anti-PAX8 (internal) Antibody

Cat. Num. AAA420181

• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: PAX8; Polyclonal Antibody; Goat anti-PAX8 (internal) Antibody; Goat Anti-PAX8 (N Terminus) Antibody; paired box gene 8; HGNC: 8622; paired domain gene 8; paired box 8; PAX8 antibody; paired box gene 8 antibody; HGNC: 8622 antibody; paired domain gene 8 antibody; paired box 8 antibody; PAX8 (internal); anti-PAX8 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: AQPGSDKRKMDD
• Sequence Length: 450

• Applicable Applications for anti-PAX8 antibody: Peptide ELISA (EIA), Western Blot (WB-Trf)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Western Blot: In transfected HEK293 transiently expressing PAX8A a band of approx.55kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.5ug/ml.
• Immunogen: Peptide with sequence C-AQPGSDKRKMDD, from the internal region of the protein sequence according to NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1.
• Epitope: Internal region
• Note: This antibody is expected to recognise all reported isoforms (NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1)
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

(HEK293 overexpressing PAX8A (RC200651) and probed (mock transfection in first lane), tested by Origene.)

NCBI and Uniprot Product Information

• NCBI GI #: 5803117
• NCBI GeneID: 7849
• NCBI Accession #: NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1
• NCBI GenBank Nucleotide #: NM_003466.3
• Molecular Weight: 31,044 Da
• NCBI Official Full Name: paired box protein Pax-8 isoform PAX8A
• NCBI Official Synonym Full Names: paired box 8
• NCBI Official Symbol: PAX8
• NCBI Protein Information: paired box protein Pax-8
• UniProt Protein Name: Paired box protein Pax-8
• Protein Family: Paired box protein
• UniProt Gene Name: PAX8
• UniProt Entry Name: PAX8_HUMAN

NCBI Description

This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010]

Uniprot Description

PAX8: Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells. Interacts with WWTR1. Expressed in the excretory system, thyroid gland and Wilms tumors. 5 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 2q13

Cellular Component: nucleoplasm; nucleus

Molecular Function: DNA binding; protein binding; thyroid-stimulating hormone receptor activity; transcription factor activity

Biological Process: anatomical structure morphogenesis; central nervous system development; inner ear morphogenesis; kidney development; mesonephros development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; pronephros development; regulation of apoptosis; sulfur metabolic process; thyroid gland development; transcription from RNA polymerase II promoter; transcription, DNA-dependent; ureteric bud branching; urogenital system development

Disease: Hypothyroidism, Congenital, Nongoitrous, 2