Rabbit NRAS / N-ras Polyclonal Antibody | anti-NRAS antibody

Rabbit Polyclonal (IgG) to Human NRAS / N-ras

Cat. Num. AAA248341

• Gene Names: NRAS; NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: NRAS / N-ras; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human NRAS / N-ras; Anti-NRAS / N-ras Antibody (C-Terminus) IHC-plus; NRAS; HRAS1; NRAS1; NS6; ALPS4; Transforming protein N-Ras; N-ras; N-ras protein part 4; GTPase NRas; Human NRAS; anti-NRAS antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: N-RAS antibody is predicted to not cross-react with H-RAS.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 189

• Applicable Applications for anti-NRAS antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (1 - 2 ug/ml)
• Target Species: Human
• Immunogen Description: 15 amino acid peptide near the carboxy terminus of human N-RAS.
• Immunogen: NRAS / N-ras antibody was raised against 15 amino acid peptide near the carboxy terminus of human N-RAS.
• Antigen Modification: C-Terminus
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-NRAS / N-ras antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot analysis of N-RAS in 293 cell lysate with N-RAS antibody at (A) 1 and (B) 2 ug/ml.)

Related Product Information for anti-NRAS antibody

The N-ras oncogene is a member of the RAS gene family. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. The order of nearby genes is as follows: cen--CD2--NGFB--NRAS--tel. The mammalian ras gene family consists of the harvey and kirsten ras genes (c-Hras1 and c-Kras2), an inactive pseudogene of each (c-Hras2 and c-Kras1) and the N-ras gene. They differ significantly only in the C-terminal 40 amino acids.

Product Categories/Family for anti-NRAS antibody

Family: Ras GTPase superfamily IPR001806; Subfamily: RAS oncogene

NCBI and Uniprot Product Information

• NCBI GI #: 4505451
• NCBI GeneID: 4893
• NCBI Accession #: NP_002515.1
• NCBI GenBank Nucleotide #: NM_002524.4
• UniProt Accession #: P01111; Q14971; Q15104; Q15282
• Molecular Weight: 21,229 Da
• NCBI Official Full Name: GTPase NRas
• NCBI Official Synonym Full Names: neuroblastoma RAS viral (v-ras) oncogene homolog
• NCBI Official Symbol: NRAS
• NCBI Official Synonym Symbols: NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
• NCBI Protein Information: GTPase NRas; N-ras protein part 4; transforming protein N-Ras; v-ras neuroblastoma RAS viral oncogene homolog
• UniProt Protein Name: GTPase NRas
• Protein Family: GTPase
• UniProt Gene Name: NRAS
• UniProt Synonym Gene Names: HRAS1
• UniProt Entry Name: RASN_HUMAN

NCBI Description

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]

Uniprot Description

NRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family.

Protein type: G protein; Motility/polarity/chemotaxis; G protein, monomeric, Ras; Oncoprotein; G protein, monomeric

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: Golgi membrane; membrane; plasma membrane

Molecular Function: GTP binding; protein complex binding

Biological Process: epidermal growth factor receptor signaling pathway; regulation of long-term neuronal synaptic plasticity; negative regulation of skeletal muscle development; axon guidance; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; activation of MAPKK activity; MAPKKK cascade; regulation of synaptic transmission, GABAergic; positive regulation of Rac protein signal transduction; negative regulation of cell differentiation; small GTPase mediated signal transduction; positive regulation of cell proliferation; Ras protein signal transduction; insulin receptor signaling pathway; striated muscle cell differentiation; visual learning; innate immune response; negative regulation of neuron apoptosis; blood coagulation; actin cytoskeleton organization and biogenesis; vascular endothelial growth factor receptor signaling pathway; leukocyte migration

Disease: Thyroid Carcinoma, Follicular; Ras-associated Autoimmune Leukoproliferative Disorder; Noonan Syndrome 6; Schimmelpenning-feuerstein-mims Syndrome; Colorectal Cancer; Melanocytic Nevus Syndrome, Congenital; Melanosis, Neurocutaneous; Nevus, Epidermal