Human GTPase NRas (NRAS) ELISA Kit | NRAS elisa kit

Human GTPase NRas (NRAS) ELISA Kit

Cat. Num. AAA7222368

• Gene Names: NRAS; NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
• Reactivity: Human
• Synonyms: GTPase NRas (NRAS); Human GTPase NRas (NRAS) ELISA Kit; NRAS elisa kit

• Reactivity: Human

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NRAS elisa kit

Intended Uses: This NR2F2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanNR2F2. This ELISA kit for research use only!

Principle of the Assay: NR2F2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NR2F2 antibody and an NR2F2-HRP conjugate. The assay sample and buffer are incubated together with NR2F2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NR2F2 concentration since NR2F2 from samples and NR2F2-HRP conjugate compete for the anti-NR2F2 antibody binding site. Since the number of sites is limited, as more sites are occupied by NR2F2 from the sample, fewer sites are left to bind NR2F2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NR2F2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for NRAS elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 4505451
• NCBI GeneID: 4893
• NCBI Accession #: NP_002515.1
• NCBI GenBank Nucleotide #: NM_002524.4
• Molecular Weight: 21,229 Da
• NCBI Official Full Name: GTPase NRas
• NCBI Official Synonym Full Names: neuroblastoma RAS viral (v-ras) oncogene homolog
• NCBI Official Symbol: NRAS
• NCBI Official Synonym Symbols: NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
• NCBI Protein Information: GTPase NRas; N-ras protein part 4; transforming protein N-Ras; v-ras neuroblastoma RAS viral oncogene homolog
• UniProt Protein Name: GTPase NRas
• Protein Family: GTPase
• UniProt Gene Name: NRAS
• UniProt Synonym Gene Names: HRAS1
• UniProt Entry Name: RASN_HUMAN

NCBI Description

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]

Uniprot Description

NRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family.

Protein type: Motility/polarity/chemotaxis; Oncoprotein; G protein, monomeric, Ras; G protein; G protein, monomeric

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: Golgi membrane; membrane; plasma membrane

Molecular Function: GTP binding; protein complex binding

Biological Process: epidermal growth factor receptor signaling pathway; regulation of long-term neuronal synaptic plasticity; negative regulation of skeletal muscle development; axon guidance; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; activation of MAPKK activity; MAPKKK cascade; regulation of synaptic transmission, GABAergic; positive regulation of Rac protein signal transduction; negative regulation of cell differentiation; small GTPase mediated signal transduction; positive regulation of cell proliferation; Ras protein signal transduction; insulin receptor signaling pathway; visual learning; striated muscle cell differentiation; innate immune response; negative regulation of neuron apoptosis; actin cytoskeleton organization and biogenesis; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration

Disease: Thyroid Carcinoma, Follicular; Ras-associated Autoimmune Leukoproliferative Disorder; Noonan Syndrome 6; Schimmelpenning-feuerstein-mims Syndrome; Colorectal Cancer; Melanocytic Nevus Syndrome, Congenital; Melanosis, Neurocutaneous; Nevus, Epidermal