Goat NRAS / N-ras Polyclonal Antibody | anti-NRAS antibody

Goat Polyclonal to Human NRAS / N-ras

Cat. Num. AAA242079

• Gene Names: NRAS; NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
• Reactivity: Reactivity:; Human, Mouse, Bat, Bovine, Dog, Horse, Pig (tested or 100% immunogen sequence identity); Predicted Reactivity: ; Monkey, Rat, Guinea pig, Hamster (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: NRAS / N-ras; Polyclonal Antibody; Goat Polyclonal to Human NRAS / N-ras; Anti-NRAS / N-ras Antibody (Internal) IHC-plus; NRAS; HRAS1; NRAS1; NS6; ALPS4; Transforming protein N-Ras; N-ras; N-ras protein part 4; GTPase NRas; Human NRAS; anti-NRAS antibody

• Host: Goat
• Reactivity: Reactivity:; Human, Mouse, Bat, Bovine, Dog, Horse, Pig (tested or 100% immunogen sequence identity); Predicted Reactivity: ; Monkey, Rat, Guinea pig, Hamster (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human NRAS.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 189

• Applicable Applications for anti-NRAS antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: Usage Summary: ; Immunohistochemistry: MBS242079 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS242079 was determined to be 2.5 ug/ml.; ; Uses:; IHC - Paraffin (2.5 µg/ml), Western blot (1:32000) & (0.01 - 0.03 ug/ml), ELISA (1:32000) (Optimal dilution to be determined by the researcher).
• Target Species: Human
• Immunogen Description: Synthetic peptide C-RMKKLNSSDDGTQ from an internal region of human NRAS (NP_002515.1). Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Mouse, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum (100%).
• Immunogen Type: NRAS / N-ras antibody was raised against synthetic peptide C-RMKKLNSSDDGTQ from an internal region of human NRAS (NP_002515.1). Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Mouse, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum (100%); Marmoset, Rat, Hamster, Guinea pig (92%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-NRAS antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Antibody (0.01 ug/ml) staining of A431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-NRAS antibody

The N-ras oncogene is a member of the RAS gene family. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. The order of nearby genes is as follows: cen--CD2--NGFB--NRAS--tel. The mammalian ras gene family consists of the harvey and kirsten ras genes (c-Hras1 and c-Kras2), an inactive pseudogene of each (c-Hras2 and c-Kras1) and the N-ras gene. They differ significantly only in the C-terminal 40 amino acids. These ras genes have GTP/GDP binding and GTPase activity, and their normal function may be as G-like regulatory proteins involved in the normal control of cell growth. Mutations which change amino acid residues 12, 13 or 61 activate the potential of N-ras to transform cultured cells and are implicated in a variety of human tumors. The N-ras gene specifies two main transcripts of 2Kb and 4.3Kb. The difference between the two transcripts is a simple extension through the termination site of the 2Kb transcript. The N-ras gene consists of seven exons (-I, I, II, III, IV, V, VI). The smaller 2Kb transcript contains the VIa exon, and the larger 4.3Kb transcript contains the VIb exon which is just a longer form of the VIa exon. Both transcripts encode identical proteins as they differ only the 3' untranslated region. The sequence of the shorter 2Kb transcript is presented here. The 4.3 Kb transcript sequence is not available.

Product Categories/Family for anti-NRAS antibody

Family: Ras GTPase superfamily IPR001806; Subfamily: RAS oncogene

NCBI and Uniprot Product Information

• NCBI GI #: 4505451
• NCBI GeneID: 4893
• NCBI Accession #: NP_002515.1
• NCBI GenBank Nucleotide #: NM_002524.4
• UniProt Accession #: P01111; Q14971; Q15104; Q15282
• NCBI Official Full Name: GTPase NRas
• NCBI Official Synonym Full Names: neuroblastoma RAS viral (v-ras) oncogene homolog
• NCBI Official Symbol: NRAS
• NCBI Official Synonym Symbols: NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
• NCBI Protein Information: GTPase NRas; N-ras protein part 4; transforming protein N-Ras; v-ras neuroblastoma RAS viral oncogene homolog
• UniProt Protein Name: GTPase NRas
• Protein Family: GTPase
• UniProt Gene Name: NRAS
• UniProt Synonym Gene Names: HRAS1
• UniProt Entry Name: RASN_HUMAN

NCBI Description

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]

Uniprot Description

NRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family.

Protein type: G protein, monomeric; G protein, monomeric, Ras; Motility/polarity/chemotaxis; Oncoprotein; G protein

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: Golgi membrane; membrane; plasma membrane

Molecular Function: GTP binding; protein complex binding

Biological Process: regulation of long-term neuronal synaptic plasticity; epidermal growth factor receptor signaling pathway; axon guidance; negative regulation of skeletal muscle development; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; activation of MAPKK activity; MAPKKK cascade; regulation of synaptic transmission, GABAergic; positive regulation of Rac protein signal transduction; negative regulation of cell differentiation; small GTPase mediated signal transduction; Ras protein signal transduction; positive regulation of cell proliferation; insulin receptor signaling pathway; striated muscle cell differentiation; visual learning; innate immune response; negative regulation of neuron apoptosis; actin cytoskeleton organization and biogenesis; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration

Disease: Thyroid Carcinoma, Follicular; Ras-associated Autoimmune Leukoproliferative Disorder; Noonan Syndrome 6; Schimmelpenning-feuerstein-mims Syndrome; Melanocytic Nevus Syndrome, Congenital; Colorectal Cancer; Melanosis, Neurocutaneous; Nevus, Epidermal