KLRK1 recombinant protein

KLRK1, 73-216aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205497

• Gene Names: KLRK1; KLR; CD314; NKG2D; NKG2-D; D12S2489E
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: KLRK1; 73-216aa; Human; His tag; E Coli (Denatured); NKG2-D type II integral membrane protein; CD314; D12S2489E; KLR; NKG2-D; NKG2D; KLRK1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol 0.4M urea
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMIWSAVF LNSLFNQEVQ IPLTESYCGP CPKNWICYKN NCYQFFDESK NWYESQASCM SQNASLLKVY SKEDQDLLKL VKSYHWMGLV HIPTNGSWQW EDGSILSPNL LTIIEMQKGD CALYASSFKG YIENCSTPNT YICMQRTV
• Sequence Length: 216

• Applicable Applications for KLRK1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for KLRK1 recombinant protein

KLRK1 is an activating receptor that has recently generated considerable interest. The most intriguing of these are a pair of closely related proteins called MICA and MICB. These are cell-surface molecules distantly related to MHC class I proteins, and the genes possess elements of heat shock promoters. MICA and MICB, therefore, are expressed during cell stress and are up-regulated in tumor cells and during viral infections. This receptor-ligand combination may play a critical role in the immune response to a variety of pathologies. Recombinant human KLRK1 protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for KLRK1 recombinant protein

Immunology

References

Guerra N. et al. (2008) Immunity. 28:571-580.; Ravetch JV. et al. (2000) Science. 290:84-89.;

NCBI and Uniprot Product Information

• NCBI GI #: 169234653
• NCBI GeneID: 22914
• NCBI Accession #: NP_031386
• NCBI GenBank Nucleotide #: NM_007360.3
• UniProt Accession #: P26718; Q9NR41; A8K7K5; A8K7P4
• Molecular Weight: 19.2 kDa (168aa)
• NCBI Official Full Name: NKG2-D type II integral membrane protein
• NCBI Official Synonym Full Names: killer cell lectin like receptor K1
• NCBI Official Symbol: KLRK1
• NCBI Official Synonym Symbols: KLR; CD314; NKG2D; NKG2-D; D12S2489E
• NCBI Protein Information: NKG2-D type II integral membrane protein
• UniProt Protein Name: NKG2-D type II integral membrane protein
• Protein Family: NKG2-D type II integral membrane protein
• UniProt Gene Name: KLRK1
• UniProt Synonym Gene Names: D12S2489E; NKG2D

NCBI Description

Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells. This gene encodes a member of the NKG2 family. The encoded transmembrane protein is characterized by a type II membrane orientation (has an extracellular C terminus) and the presence of a C-type lectin domain. It binds to a diverse family of ligands that include MHC class I chain-related A and B proteins and UL-16 binding proteins, where ligand-receptor interactions can result in the activation of NK and T cells. The surface expression of these ligands is important for the recognition of stressed cells by the immune system, and thus this protein and its ligands are therapeutic targets for the treatment of immune diseases and cancers. Read-through transcription exists between this gene and the upstream KLRC4 (killer cell lectin-like receptor subfamily C, member 4) family member in the same cluster. [provided by RefSeq, Dec 2010]

Uniprot Description

Function as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8+ T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA, MICB, RAET1E, RAET1G, RAET1L/ULBP6, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4.