Goat NR1I2 / PXR / PAR Polyclonal Antibody | anti-NR1I2 antibody

Goat Polyclonal to Human NR1I2 / PXR / PAR

Cat. Num. AAA243174

• Gene Names: NR1I2; BXR; PAR; PRR; PXR; SAR; SXR; ONR1; PAR1; PAR2; PARq
• Reactivity: Gibbon, Gorilla, Human; Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: NR1I2 / PXR / PAR; Polyclonal Antibody; Goat Polyclonal to Human NR1I2 / PXR / PAR; Anti-NR1I2 / PXR / PAR Antibody (Internal) IHC-plus; NR1I2; BXR; Orphan nuclear receptor PXR; Orphan nuclear receptor PAR1; PARq; PRR; PXR; Pregnane X receptor; ONR1; SAR; SXR; Human NR1I2; PAR; anti-NR1I2 antibody

• Host: Goat
• Reactivity: Gibbon, Gorilla, Human; Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human NR1I2 / PXR. This antibody is expected to recognize all reported isoforms (NP_071285.1; NP_148934.1; NP_003880.3).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 434

• Applicable Applications for anti-NR1I2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:4000), IHC-P (3.75 ug/ml), WB (1 - 3 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:4000. Western Blot: Approx. 48kD band observed in lysates of cell line HepG2 (calculated MW of 49.8kD according to NP_003880.3). Recommended concentration: 1-3 ug/ml. An additional band of unknown id...
• Target Species: Human
• Immunogen Description: Synthetic peptide EQFAITLKSYIECNR from an internal region of human NR1I2 / PXR (NP_003880.3; NP_071285.1; NP_148934.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey, Marmoset (93%); Horse, Pig (87%); Panda, Dog, Rabbit (80%).
• Immunogen Type: Synthetic peptide
• Immunogen: NR1I2 / PXR / PAR antibody was raised against synthetic peptide EQFAITLKSYIECNR from an internal region of human NR1I2 / PXR (NP_003880.3; NP_071285.1; NP_148934.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey, Marmoset (93%); Horse, Pig (87%); Panda, Dog, Rabbit (80%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-PXR antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Western Blot (WB)

(Antibody (1 ug/ml) staining of HepG2 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-NR1I2 antibody

Pregnane X receptor (PXR), a NR1 Thyroid Hormone-Like Receptor, has been shown to mediate the effects of steroid hormones, including progesterone, glucocorticoid, and pregnenolone, and effects of xenobiotics, through the cytochrome P-450 3A gene family (CYP3A). PXR protects the body from hepatotoxicity of secondary bile acids such as lithocholic acid (LCA) by inducing expression of the hydroxylating cytochrome P450 enzyme CYP3A and promoting detoxification.

Product Categories/Family for anti-NR1I2 antibody

Family: NHR; Subfamily: NR1 Thyroid hormone-like

NCBI and Uniprot Product Information

• NCBI GI #: 34398348
• NCBI GeneID: 8856
• NCBI Accession #: NP_003880.3
• NCBI GenBank Nucleotide #: NM_003889.3
• UniProt Accession #: O75469; Q006P5; Q008C8; Q96AC7; Q9UJ22; Q9UJ23; Q9UJ24; Q9UJ25; Q9UJ26; Q9UJ27; Q9UNW4
• Molecular Weight: 53,899 Da
• NCBI Official Full Name: nuclear receptor subfamily 1 group I member 2 isoform 1
• NCBI Official Synonym Full Names: nuclear receptor subfamily 1, group I, member 2
• NCBI Official Symbol: NR1I2
• NCBI Official Synonym Symbols: BXR; PAR; PRR; PXR; SAR; SXR; ONR1; PAR1; PAR2; PARq
• NCBI Protein Information: nuclear receptor subfamily 1 group I member 2; pregnane X receptor; orphan nuclear receptor PXR; orphan nuclear receptor PAR1; steroid and xenobiotic receptor; pregnane X nuclear receptor variant 2
• UniProt Protein Name: Nuclear receptor subfamily 1 group I member 2
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR1I2
• UniProt Synonym Gene Names: PXR; SXR
• UniProt Entry Name: NR1I2_HUMAN

NCBI Description

This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

PXR: Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes. Heterodimer with RXR. Interacts with NCOA1. Expressed in liver, colon and small intestine. Belongs to the nuclear hormone receptor family. NR1 subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 3q12-q13.3

Cellular Component: nucleoplasm

Molecular Function: ligand-dependent nuclear receptor activity; protein binding; zinc ion binding; transcription coactivator activity; steroid hormone receptor activity; drug binding

Biological Process: steroid metabolic process; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; xenobiotic metabolic process; drug export; positive regulation of transcription, DNA-dependent; exogenous drug catabolic process; gene expression; xenobiotic transport; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; signal transduction; negative regulation of transcription, DNA-dependent