Rat ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) ELISA Kit | ATP2A2 elisa kit

Rat ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) ELISA Kit

Cat. Num. AAA2098091

• Gene Names: ATP2A2; DD; DAR; ATP2B; SERCA2
• Reactivity: Rat
• Synonyms: ATPase; Ca++ Transporting; Cardiac Muscle; Slow Twitch 2 (ATP2A2); Rat ATPase; Slow Twitch 2 (ATP2A2) ELISA Kit; ATP2B; DAR; SERCA2; Calcium pump 2; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; Calcium-transporting ATPase sarcoplasmic reticulum type; slow twitch skeletal muscle; ATP2A2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2).; No significant cross-reactivity or interference between ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) and analogues was observed.
• Sequence Length: 1042

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.065ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATP2A2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ATP2A2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 23272583
• NCBI GeneID: 488
• Molecular Weight: 109,734 Da
• NCBI Official Full Name: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
• NCBI Official Synonym Full Names: ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2
• NCBI Official Symbol: ATP2A2
• NCBI Official Synonym Symbols: DD; DAR; ATP2B; SERCA2
• NCBI Protein Information: sarcoplasmic/endoplasmic reticulum calcium ATPase 2
• UniProt Protein Name: Sarcoplasmic/endoplasmic reticulum calcium ATPase 2
• Protein Family: Sarcoplasmic/endoplasmic reticulum calcium ATPase
• UniProt Gene Name: ATP2A2
• UniProt Synonym Gene Names: ATP2B; SERCA2; SR Ca(2+)-ATPase 2

NCBI Description

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2008]

Uniprot Description

SERCA2: an intracellular pump located in the sarcoplasmic or endoplasmic reticula of muscle cells. Possesses a Ca(2+)-ATPase activity. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Reversibly inhibited by phospholamban (PLN) at low calcium concentrations. Dephosphorylated PLN decreases the apparent affinity of the ATPase for calcium. This inhibition is regulated by the phosphorylation of PLN. Two alternatively spliced isoforms have been described.

Protein type: Cell adhesion; EC 3.6.3.8; Endoplasmic reticulum; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ion channel

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; extrinsic component of cytoplasmic side of plasma membrane; integral component of plasma membrane; membrane; nucleoplasm; perinuclear region of cytoplasm; protein complex; sarcoplasmic reticulum; sarcoplasmic reticulum membrane; vesicle membrane

Molecular Function: ATP binding; calcium ion binding; calcium-transporting ATPase activity; calcium-transporting ATPase activity involved in regulation of cardiac muscle cell membrane potential; enzyme binding; lutropin-choriogonadotropic hormone receptor binding; protein binding; S100 protein binding

Biological Process: calcium ion import into sarcoplasmic reticulum; cardiac muscle hypertrophy in response to stress; cell adhesion; cellular response to oxidative stress; endoplasmic reticulum calcium ion homeostasis; epidermis development; ER-nuclear signaling pathway; negative regulation of heart contraction; organelle organization and biogenesis; positive regulation of endoplasmic reticulum calcium ion concentration; positive regulation of heart rate; regulation of cardiac muscle cell action potential involved in regulation of contraction; regulation of cardiac muscle cell membrane potential; regulation of cardiac muscle contraction by calcium ion signaling; regulation of the force of heart contraction; sarcoplasmic reticulum calcium ion transport; T-tubule organization; transition between fast and slow fiber

Disease: Acrokeratosis Verruciformis; Darier-white Disease