Rabbit anti-Mouse, Rat PXR Polyclonal Antibody | anti-PXR antibody

PXR Polyclonal Antibody

Cat. Num. AAA9433096

• Gene Names: NR1I2; BXR; PAR; PRR; PXR; SAR; SXR; ONR1; PAR1; PAR2; PARq
• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity purification
• Synonyms: PXR; Polyclonal Antibody; PXR Polyclonal Antibody; NR1I2; BXR; ONR1; PAR; PAR1; PAR2; PARq; PRR; SAR; SXR; nuclear receptor subfamily 1 group I member 2; anti-PXR antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 50% glycerol, pH7.4

• Applicable Applications for anti-PXR antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant protein of human PXR
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PXR antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of C6 cells using PXR Polyclonal Antibody.)

Related Product Information for anti-PXR antibody

This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized.

Product Categories/Family for anti-PXR antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 34398348
• NCBI GeneID: 8856
• NCBI Accession #: NP_003880.3
• NCBI GenBank Nucleotide #: NM_003889.3
• UniProt Accession #: O75469; Q006P5; Q008C8; Q96AC7; Q9UJ22; Q9UJ23; Q9UJ24; Q9UJ25; Q9UJ26; Q9UJ27; Q9UNW4
• Molecular Weight: 49,762 Da
• NCBI Official Full Name: nuclear receptor subfamily 1 group I member 2 isoform 1
• NCBI Official Synonym Full Names: nuclear receptor subfamily 1, group I, member 2
• NCBI Official Symbol: NR1I2
• NCBI Official Synonym Symbols: BXR; PAR; PRR; PXR; SAR; SXR; ONR1; PAR1; PAR2; PARq
• NCBI Protein Information: nuclear receptor subfamily 1 group I member 2; pregnane X receptor; orphan nuclear receptor PXR; orphan nuclear receptor PAR1; steroid and xenobiotic receptor; pregnane X nuclear receptor variant 2
• UniProt Protein Name: Nuclear receptor subfamily 1 group I member 2
• UniProt Gene Name: NR1I2
• UniProt Synonym Gene Names: PXR; SXR
• UniProt Entry Name: NR1I2_HUMAN

NCBI Description

This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

PXR: Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes. Heterodimer with RXR. Interacts with NCOA1. Expressed in liver, colon and small intestine. Belongs to the nuclear hormone receptor family. NR1 subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 3q12-q13.3

Cellular Component: nucleoplasm

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; zinc ion binding; transcription coactivator activity; steroid hormone receptor activity; drug binding

Biological Process: steroid metabolic process; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; positive regulation of transcription, DNA-dependent; drug export; signal transduction; xenobiotic metabolic process; exogenous drug catabolic process; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; gene expression; xenobiotic transport; negative regulation of transcription, DNA-dependent