Rabbit anti-Human NR0B11 Polyclonal Antibody | anti-NR0B11 antibody

NR0B11, CT (Nuclear Receptor Subfamily 0 Group B Member 1, Nuclear Receptor DAX-1, DSS-AHC Critical Region On The X Chromosome Protein 1, DAX, AHC, DAX1) (FITC)

Cat. Num. AAA6490686

• Gene Names: NR0B1; AHC; AHX; DSS; GTD; HHG; AHCH; DAX1; DAX-1; NROB1; SRXY2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: NR0B11; Polyclonal Antibody; CT (Nuclear Receptor Subfamily 0 Group B Member 1; Nuclear Receptor DAX-1; DSS-AHC Critical Region On The X Chromosome Protein 1; DAX; AHC; DAX1) (FITC); anti-NR0B11 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NROB1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-NR0B11 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human NROB1 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NROB1 (arrow) using rabbit polyclonal NROB1 Antibody (Human C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NROB1 gene (Lane 2) (Origene Technologies).)

Product Categories/Family for anti-NR0B11 antibody

Antibodies; Receptors

References

1. Calliari, L.E., Genet. Mol. Res. 6(2), 177-183 (2007). 2. Kinsey, M., Mol. Cancer Res. 4(11), 851-859 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 5016090
• NCBI GeneID: 190
• NCBI Accession #: NP_000466
• NCBI GenBank Nucleotide #: NM_000475.4
• UniProt Accession #: P51843; Q96F69
• Molecular Weight: 43,590 Da
• NCBI Official Full Name: nuclear receptor subfamily 0 group B member 1
• NCBI Official Synonym Full Names: nuclear receptor subfamily 0, group B, member 1
• NCBI Official Symbol: NR0B1
• NCBI Official Synonym Symbols: AHC; AHX; DSS; GTD; HHG; AHCH; DAX1; DAX-1; NROB1; SRXY2
• NCBI Protein Information: nuclear receptor subfamily 0 group B member 1
• UniProt Protein Name: Nuclear receptor subfamily 0 group B member 1
• UniProt Gene Name: NR0B1
• UniProt Synonym Gene Names: AHC; DAX1
• UniProt Entry Name: NR0B1_HUMAN

NCBI Description

This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism. [provided by RefSeq, Jul 2008]

Uniprot Description

NR0B1: orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency. Homodimerizes with STF-1, NR5A2, NR0B2 and with COPS2. Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus. Homodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with STF-1 and NROB2 through its N-terminal LXXLL motifs. Defects in NR0B1 are the cause of congenital X-linked adrenal hypoplasia. Two alternatively spliced human isoforms have been described.

Protein type: Nuclear receptor

Chromosomal Location of Human Ortholog: Xp21.3

Cellular Component: nucleoplasm; polysomal ribosome; membrane; cytoplasm; nucleus

Molecular Function: protein domain specific binding; DNA hairpin binding; protein binding; ligand-dependent nuclear receptor activity; protein homodimerization activity; DNA binding; AF-2 domain binding; RNA binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription corepressor activity; transcription factor binding; steroid hormone receptor binding

Biological Process: transcription initiation from RNA polymerase II promoter; hypothalamus development; negative regulation of steroid hormone receptor signaling pathway; intracellular receptor-mediated signaling pathway; gonad development; adrenal gland development; negative regulation of transcription factor activity; male gonad development; Sertoli cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell differentiation; protein localization; Leydig cell differentiation; pituitary gland development; male sex determination; steroid hormone mediated signaling; spermatogenesis; gene expression; negative regulation of transcription, DNA-dependent; steroid biosynthetic process

Disease: 46,xy Sex Reversal 2; Adrenal Hypoplasia, Congenital