Immunohistochemistry (IHC)
(MBS9604337 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human NPY4R Polyclonal Antibody | anti-NPY4R antibody
NPY4R Antibody
Gene Names
NPY4R; Y4; PP1; PPYR1; NPY4-R
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Peptide affinity purification
Synonyms
NPY4R; Polyclonal Antibody; NPY4R Antibody; MGC116897; Neuropeptide Y receptor type 4; NPY4 R; NPY4-R; Npy4r; NPY4R_HUMAN; Pancreatic polypeptide receptor 1; PP 1; PP1; PPY R1; PPYR 1; PPYR1; Y4; anti-NPY4R antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
NPY4R antibody detects endogenous levels of total NPY4R
Purity/Purification
Peptide affinity purification
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
375
Applicable Applications for anti-NPY4R antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA: 1:20000-1:40000
The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
IHC: 1:50-1:200
ELISA: 1:20000-1:40000
The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunogen
A synthesized peptide derived from human NPY4R, corresponding to a region within N-terminal amino acids.
Conjugation
Unconjugated
Immunohistochemistry (IHC)
(MBS9604337 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9604337 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of NPY4R expression in K562 cell line lysates, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of NPY4R expression in K562 cell line lysates, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-NPY4R antibody
Description: Receptor for neuropeptide Y and peptide YY. The rank order of affinity of this receptor for pancreatic polypeptides is PP, PP (2-36) and [Ile-31, Gln-34] PP > [Pro-34] PYY > PYY and [Leu-31, Pro-34] NPY > NPY > PYY (3-36) and NPY (2-36) > PP (13-36) > PP (31-36) > NPY free acid.
Similarity: Belongs to the G-protein coupled receptor 1 family.
Similarity: Belongs to the G-protein coupled receptor 1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42 kDa
NCBI Official Full Name
neuropeptide Y receptor type 4
NCBI Official Synonym Full Names
neuropeptide Y receptor Y4
NCBI Official Symbol
NPY4R
NCBI Official Synonym Symbols
Y4; PP1; PPYR1; NPY4-R
NCBI Protein Information
neuropeptide Y receptor type 4
UniProt Protein Name
Neuropeptide Y receptor type 4
Protein Family
UniProt Gene Name
NPY4R
UniProt Synonym Gene Names
PPYR1; NPY4-R; PP1
Uniprot Description
Receptor for neuropeptide Y and peptide YY. The rank order of affinity of this receptor for pancreatic polypeptides is PP, PP (2-36) and [Ile-31, Gln-34] PP > [Pro-34] PYY > PYY and [Leu-31, Pro-34] NPY > NPY > PYY (3-36) and NPY (2-36) > PP (13-36) > PP (31-36) > NPY free acid.
Research Articles on NPY4R
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Product Notes
The NPY4R npy4r (Catalog #AAA9604337) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NPY4R Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NPY4R can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA: 1:20000-1:40000 The optimal dilutions should be determined by the end user. IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the NPY4R npy4r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NPY4R, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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