Rabbit BEST1 Polyclonal Antibody | anti-BEST1 antibody

BEST1 Polyclonal Antibody

Cat. Num. AAA3011315

• Gene Names: BEST1; ARB; BMD; BEST; RP50; VMD2; TU15B; Best1V1Delta2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: BEST1; Polyclonal Antibody; BEST1 Polyclonal Antibody; ARB; BEST 1; BEST; Best disease; Best macular dystrophy; BEST1_HUMAN; Best1V1Delta2; Bestrophin 1; Bestrophin-1; Bestrophin1; BMD; mBest1; RP50; TU15B; Vitelliform macular dystrophy 2; Vitelliform macular dystrophy; Vitelliform macular dystrophy protein 2; VMD 2; VMD2; anti-BEST1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: BEST1 polyclonal antibody detects endogenous levels of BEST1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 604

• Applicable Applications for anti-BEST1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant full length Human BEST1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of BEST1 polyclonal antibody)

Related Product Information for anti-BEST1 antibody

-The retinal pigment epithelium (RPE) and choroid represent a differentiated system of the eye that sustains normal retinal health and function. Best vitelliform macular dystrophy, known as Best disease, is an early-onset autosomal dominant condition in which accumulation of lipofuscin-like material within and beneath the RPE leads to progressive loss of central vision. The lipofuscin-like material in the macular area appears as a yellow mass like the yolk of an egg that later becomes darker and irregular in color, a process known as "scrambling the egg". Best disease is frequently a reflection of mutations in the Bestrophin gene, which encodes a protein containing four putative transmembrane domains and localizes to the basolateral plasma membrane of RPE cells. Human Bestrophin forms oligomeric chloride channels that are sensitive to intracellular calcium. Missense mutations at the Bestrophin locus reduces or abolishes Bestrophin protein mediated membrane current. The human Bestrophin gene encodes a 585 amino acid protein.

NCBI and Uniprot Product Information

• NCBI GI #: 212720889
• NCBI GeneID: 7439
• NCBI Accession #: NP_001132915.1
• NCBI GenBank Nucleotide #: NP_001132915.1
• UniProt Accession #: O76090
• Molecular Weight: ~ 68kDa
• NCBI Official Full Name: bestrophin-1 isoform 2
• NCBI Official Synonym Full Names: bestrophin 1
• NCBI Official Symbol: BEST1
• NCBI Official Synonym Symbols: ARB; BMD; BEST; RP50; VMD2; TU15B; Best1V1Delta2
• NCBI Protein Information: bestrophin-1
• UniProt Protein Name: Bestrophin-1
• Protein Family: Bestrophin
• UniProt Gene Name: BEST1
• UniProt Synonym Gene Names: VMD2
• UniProt Entry Name: BEST1_HUMAN

NCBI Description

This gene encodes a member of the bestrophin gene family. This small gene family is characterized by proteins with a highly conserved N-terminus with four to six transmembrane domains. Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels. Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue. Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies. Alternative splicing results in multiple variants encoding distinct isoforms.[provided by RefSeq, Nov 2008]