Rabbit Npr2 Polyclonal Antibody | anti-Npr2 antibody

Mouse Npr2 Antibody (Center)

Cat. Num. AAA9210430

• Gene Names: Npr2; cn; pwe; GC-B; GC-B2; GC-B3; mNPR-B
• Reactivity: Mouse (Predicted Reactivity: Rat)
• Applications: Western Blot, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Npr2; Polyclonal Antibody; Mouse Npr2 Antibody (Center); Atrial natriuretic peptide receptor 2; Atrial natriuretic peptide receptor type B; ANP-B; ANPR-B; NPR-B; Guanylate cyclase B; GC-B; anti-Npr2 antibody

• Host: Rabbit
• Reactivity: Mouse (Predicted Reactivity: Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Mouse Npr2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 480-509 amino acids from the Central region of mouse Npr2.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 480-509
• Sequence Length: 1047

• Applicable Applications for anti-Npr2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: MOUSE
• Function: Receptor for the C-type natriuretic peptide NPPC/CNP hormone. Has guanylate cyclase activity upon binding of its ligand. May play a role in the regulation of skeletal growth.
• Cellular Location: Membrane; Single-pass type I membrane protein
• Tissue Location: Widely expressed. Expressed in the columnar proliferating and prehypertrophic chondrocyte layers of the tibia
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-Mouse Npr2 Antibody (Center) at 1:1000 dilution + mouse heart lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 117 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-Npr2 antibody

Receptor for the C-type natriuretic peptide NPPC/CNP hormone. Has guanylate cyclase activity upon binding of its ligand. May play a role in the regulation of skeletal growth.

Product Categories/Family for anti-Npr2 antibody

Cardiovascular; Signal Transduction

References

Zhang, M., et al. Science 330(6002):366-369(2010)
Sogawa, C., et al. Am. J. Pathol. 177(2):822-828(2010)
Zhao, Z., et al. Proc. Natl. Acad. Sci. U.S.A. 106(42):18016-18021(2009)
Schmidt, H., et al. Proc. Natl. Acad. Sci. U.S.A. 106(39):16847-16852(2009)
Yoder, A.R., et al. Peptides 29(9):1575-1581(2008)

NCBI and Uniprot Product Information

• NCBI GI #: 28916675
• NCBI GeneID: 230103
• NCBI Accession #: NP_776149.1
• NCBI GenBank Nucleotide #: NM_173788.3
• UniProt Accession #: Q6VVW5; Q6VVW3; Q6VVW4; Q8CGA9; B1AWI8
• Molecular Weight: 117060
• NCBI Official Full Name: atrial natriuretic peptide receptor 2
• NCBI Official Synonym Full Names: natriuretic peptide receptor 2
• NCBI Official Symbol: Npr2
• NCBI Official Synonym Symbols: cn; pwe; GC-B; GC-B2; GC-B3; mNPR-B
• NCBI Protein Information: atrial natriuretic peptide receptor 2
• UniProt Protein Name: Atrial natriuretic peptide receptor 2
• Protein Family: Nitrogen permease regulator
• UniProt Gene Name: Npr2
• UniProt Synonym Gene Names: ANP-B; ANPR-B; NPR-B; GC-B
• UniProt Entry Name: ANPRB_MOUSE

Uniprot Description

ANPB: natriuretic peptide receptor B, one of two integral membrane receptors for natriuretic peptides. Contains five functional domains: an extracellular ligand-binding domain, a single membrane-spanning region, and intracellularly a protein kinase homology domain, a helical hinge region involved in oligomerization, and a carboxyl-terminal guanylyl cyclase catalytic domain. Is the primary receptor for C-type natriuretic peptide (CNP), which upon ligand binding exhibits greatly increased guanylyl cyclase activity. 21 mutations linked to acromesomelic dysplasia (skeletal malformation). 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, RGC; Protein kinase, dual-specificity (receptor); EC 4.6.1.2; Kinase, protein; Membrane protein, integral; Nucleotide Metabolism - purine; Guanylyl cyclase; Lyase; Receptor, misc.; RGC group; RGC family

Cellular Component: membrane; integral to membrane; plasma membrane

Molecular Function: identical protein binding; guanylate cyclase activity; hormone binding; phosphorus-oxygen lyase activity; GTP binding; natriuretic peptide receptor activity; peptide hormone binding; transferase activity, transferring phosphorus-containing groups; lyase activity; nucleotide binding; ATP binding; protein kinase activity

Biological Process: cGMP biosynthetic process; cyclic nucleotide biosynthetic process; ossification; receptor guanylyl cyclase signaling pathway; negative regulation of meiotic cell cycle; signal transduction; protein amino acid phosphorylation