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Western Blot (WB) (Gel: 6%SDS-PAGE Lysate: 40 μ g Lane 1-3: Jurkat£¬Hela and 293T cell Primary antibody: 1/250 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds)

Rabbit anti-Human CCNT2 Polyclonal Antibody | anti-CCNT2 antibody

CCNT2 Antibody

Gene Names
CCNT2; CYCT2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
CCNT2; Polyclonal Antibody; CCNT2 Antibody; CYCT2; anti-CCNT2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total CCNT2 protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
1.3 mg/ml (varies by lot)
Sequence Length
663
Applicable Applications for anti-CCNT2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:25-1:100
Immunogen
Synthetic peptide of human CCNT2
Immunogen Type
Peptide
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Gel: 6%SDS-PAGE Lysate: 40 μ g Lane 1-3: Jurkat£¬Hela and 293T cell Primary antibody: 1/250 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds)

Western Blot (WB) (Gel: 6%SDS-PAGE Lysate: 40 μ g Lane 1-3: Jurkat£¬Hela and 293T cell Primary antibody: 1/250 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue using at dilution 1/20.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue using at dilution 1/20.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using at dilution 1/20.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using at dilution 1/20.)
Related Product Information for anti-CCNT2 antibody
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases.Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin and its kinase partner CDK9 were found to be subunits of the transcription elongation factor p-TEFb. The p-TEFb complex containing this cyclin was reported to interact with, and act as a negative regulator of human immunodeficiency virus type 1 (HIV-1) Tat protein.
Product Categories/Family for anti-CCNT2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
905
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
73,682 Da
NCBI Official Full Name
cyclin-T2 isoform a
NCBI Official Synonym Full Names
cyclin T2
NCBI Official Symbol
CCNT2
NCBI Official Synonym Symbols
CYCT2
NCBI Protein Information
cyclin-T2
UniProt Protein Name
Cyclin-T2
Protein Family
UniProt Gene Name
CCNT2
UniProt Synonym Gene Names
CycT2
UniProt Entry Name
CCNT2_HUMAN

Uniprot Description

CCNT2: Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II). Belongs to the cyclin family. Cyclin C subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 2q21.3

Cellular Component: nucleoplasm; nucleus; transcription elongation factor complex b

Molecular Function: chromatin binding; cyclin-dependent protein kinase regulator activity; protein binding; protein serine/threonine kinase activity

Biological Process: immediate early viral mRNA transcription; late viral mRNA transcription; positive regulation of cyclin-dependent protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; regulation of cyclin-dependent protein kinase activity; RNA elongation from RNA polymerase II promoter; snRNA transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter

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Product Notes

The CCNT2 ccnt2 (Catalog #AAA9415375) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CCNT2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CCNT2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:500-1:2000 Immunohistochemistry: 1:25-1:100. Researchers should empirically determine the suitability of the CCNT2 ccnt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCNT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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