Rabbit NPM1 Polyclonal Antibody | anti-NPM1 antibody

Anti-NPM1 Antibody

Cat. Num. AAA416547

• Gene Names: NPM1; B23; NPM
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Purified
• Synonyms: NPM1; Polyclonal Antibody; Anti-NPM1 Antibody; Anti-Nucleophosmin Antibody; anti-NPM1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: nucleophosmin antibody detects endogenous levels of nucleophosmin protein. Not tested with other proteins
• Purity/Purification: Purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 1449

• Applicable Applications for anti-NPM1 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Recommended dilutions:; Peptide ELISA: 1:20,000; WB: 1:100 - 1:500; Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Synthetic peptide conjugated to KLH
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Related Product Information for anti-NPM1 antibody

This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]

References

Cochrane,A.W., Perkins,A. and Rosen,C.A Identification of sequences important in the nucleolar localization of human immunodeficiency virus Rev: relevance of nucleolar localization to functionJ. Virol. 1990:64 (2) 881-885

NCBI and Uniprot Product Information

• NCBI GI #: 262331543
• NCBI GeneID: 4869
• NCBI Accession #: NP_002511.1
• NCBI GenBank Nucleotide #: NM_002520
• Molecular Weight: 28,400 Da
• NCBI Official Full Name: Homo sapiens nucleophosmin (nucleolar phosphoprotein B23, numatrin) (NPM1), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• NCBI Official Symbol: NPM1
• NCBI Official Synonym Symbols: B23; NPM
• NCBI Protein Information: nucleophosmin; nucleolar protein NO38; nucleophosmin/nucleoplasmin family, member 1
• UniProt Protein Name: Nucleophosmin
• Protein Family: Nucleophosmin
• UniProt Gene Name: NPM1
• UniProt Entry Name: NPM_HUMAN

NCBI Description

This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]

Uniprot Description

NPM1: a nucleolar protein associated with nucleolar ribonucleoprotein structures and that binds single-stranded nucleic acids. Is a major component of template activating factor (TAF)-III. It plays a role in controlling centrosome duplication, and may function in the assembly and/or transport of the ribosome. Two alternatively spliced isoforms have been described.

Protein type: RNA-binding; Nucleolus; Translation; Oncoprotein

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: nucleoplasm; centrosome; focal adhesion; membrane; cytoplasm; nucleolus; spindle pole centrosome; cytosol; nucleus; ribonucleoprotein complex

Molecular Function: protein homodimerization activity; protein kinase inhibitor activity; histone binding; RNA binding; unfolded protein binding; transcription coactivator activity; protein kinase binding; ribosomal large subunit binding; NF-kappaB binding; protein binding; Tat protein binding; protein heterodimerization activity; ribosomal small subunit binding

Biological Process: regulation of centriole replication; viral reproduction; positive regulation of translation; centrosome cycle; cell aging; nucleocytoplasmic transport; signal transduction; DNA repair; protein oligomerization; activation of NF-kappaB transcription factor; intracellular protein transport; negative regulation of cell proliferation; nucleosome assembly; regulation of endodeoxyribonuclease activity; protein localization; DNA replication-independent nucleosome assembly at centromere; ribosome assembly; response to stress; negative regulation of apoptosis