Rabbit anti-Human, Mouse PCK1 Polyclonal Antibody | anti-PCK1 antibody

PCK1, NT (PCK1, PEPCK1, Phosphoenolpyruvate carboxykinase, cytosolic [GTP], Phosphoenolpyruvate carboxylase) (AP)

Cat. Num. AAA6331792

• Gene Names: PCK1; PEPCK1; PEPCKC; PEPCK-C
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PCK1; Polyclonal Antibody; NT (PCK1; PEPCK1; Phosphoenolpyruvate carboxykinase; cytosolic [GTP]; Phosphoenolpyruvate carboxylase) (AP); Phosphoenolpyruvate carboxylase; anti-PCK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-PCK1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48~77 amino acids from the N-terminal region of human PCK1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(The anti-PCK1 Pab is used in Western blot to detect PCK1 in mouse liver tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(PCK1 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the PCK1 antibody detected the PCK1 protein (arrow).)

Related Product Information for anti-PCK1 antibody

This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme of the encoded protein also has been characterized.

Product Categories/Family for anti-PCK1 antibody

Antibodies; Enzymes

References

Berger, K. et al. PLoS ONE. 4(3): e4671 (2009). Dunten, P., et al., J. Mol. Biol. 316(2):257-264 (2002). Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Deloukas, P., et al., Nature 414(6866):865-871 (2001). O'Brien, R.M., et al., Biochim. Biophys. Acta 1264(3):284-288 (1995). Ting, C.N., et al., Genomics 16(3):698-706 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 93138710
• NCBI GeneID: 5105
• UniProt Accession #: P35558; Q8TCA3; Q9UJD2; A8K437; B4DT64
• Molecular Weight: 622
• NCBI Official Full Name: Phosphoenolpyruvate carboxykinase, cytosolic
• NCBI Official Synonym Full Names: phosphoenolpyruvate carboxykinase 1 (soluble)
• NCBI Official Symbol: PCK1
• NCBI Official Synonym Symbols: PEPCK1; PEPCKC; PEPCK-C
• NCBI Protein Information: phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxykinase, cytosolic [GTP]; PEP carboxykinase; phosphopyruvate carboxylase; phosphoenolpyruvate carboxylase
• UniProt Protein Name: Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
• Protein Family: Protein kinase
• UniProt Gene Name: PCK1
• UniProt Synonym Gene Names: PEPCK1; PEPCK-C
• UniProt Entry Name: PCKGC_HUMAN

NCBI Description

This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

PCK1: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD). A metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait. Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Protein type: Kinase, other; Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - pyruvate; Lyase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.1.1.32

Chromosomal Location of Human Ortholog: 20q13.31

Cellular Component: cytoplasm; cytosol

Molecular Function: GDP binding; GTP binding; carboxylic acid binding; manganese ion binding; phosphoenolpyruvate carboxykinase (GTP) activity; magnesium ion binding

Biological Process: oxaloacetate metabolic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis; response to activity; drug metabolic process; glucose homeostasis; internal protein amino acid acetylation; glycerol biosynthetic process from pyruvate; response to insulin stimulus; gluconeogenesis

Disease: Phosphoenolpyruvate Carboxykinase Deficiency, Cytosolic