Mouse anti-Human MUTYH Monoclonal Antibody | anti-MUTYH antibody

MUTYH (A/G-specific Adenine DNA Glycosylase, MutY Homolog, hMYH, MYH) (PE)

Cat. Num. AAA6158948

• Gene Names: MUTYH; MYH
• Reactivity: Human
• Applications: ELISA, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MUTYH; Monoclonal Antibody; MUTYH (A/G-specific Adenine DNA Glycosylase; MutY Homolog; hMYH; MYH) (PE); anti-MUTYH antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4D10
• Specificity: Recognizes human MUTYH.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MUTYH antibody: ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa436-535 from human MUTYH (AAH03178) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: KLTYQVYGLALEGQTPVTTVPPGARWLTQEEFHTAAVSTAMKKVFRVYQGQQPGTCMGSKRSQVSSPCSRKKPRMGQQVLDNFFRSHISTDAHSLNSAAQ
• Conjugate: PE
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (36.74kD).)

Western Blot (WB)

(MUTYH monoclonal antibody Western Blot analysis of MUTYH expression in HeLa NE.)

Western Blot (WB)

(Western Blot analysis of MUTYH expression in transfected 293T cell line by MUTYH monoclonal antibody. Lane 1: MUTYH transfected lysate (59.1kD). Lane 2: Non-transfected lysate.)

Immunoprecipitation (IP)

(Immunoprecipitation of MUTYH transfected lysate using anti-MUTYH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MUTYH rabbit polyclonal antibody.)

Testing Data

(Detection limit for recombinant GST tagged MUTYH is ~1ng/ml as a capture antibody.)

Western Blot (WB)

(Western blot analysis of MUTYH over-expressed 293 cell line, cotransfected with MUTYH Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MUTYH monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Related Product Information for anti-MUTYH antibody

MYH is the eukaryotic homolog to the E. coli protein MutY. It was first identified in HeLa cells and nicks DNA replication errors, specifically A/G mismatches.

Product Categories/Family for anti-MUTYH antibody

Antibodies; Transcription Factors

References

1. Cancer-associated variants and a common polymorphism of MUTYH exhibit reduced repair of oxidative DNA damage using a GFP-based assay in mammalian cells. Raetz AG, Xie Y, Kundu S, Brinkmeyer MK, Chang C, David SS.Carcinogenesis. 2012 Sep 20. 2. Reduced expression of MUTYH with suppressive activity against mutations caused by 8-hydroxyguanine is a novel predictor of a poor prognosis in human gastric cancer. Shinmura K, Goto M, Suzuki M, Tao H, Yamada H, Igarashi H, Matsuura S, Maeda M, Konno H, Matsuda T, Sugimura H.The Journal of Pathology DOI: 10.1002 /path.2953. 3. Effects of base excision repair proteins on mutagenesis by 8-oxo-7,8-dihydroguanine (8-hydroxyguanine) paired with cytosine and adenine. Suzuki T, Harashima H, Kamiya H.DNA Repair (Amst). 2010 Feb 28. 4. Immunohistochemistry is not an accurate first step towards the molecular diagnosis of MUTYH-associated polyposis. van der Post RS, Kets CM, Ligtenberg MJ, van Krieken JH, Hoogerbrugge N.Virchows Arch. 2009 Jan;454(1):25-9. Epub 2008 Nov 25.

NCBI and Uniprot Product Information

• NCBI GI #: 13112008
• NCBI GeneID: 4595
• Molecular Weight: 57,446 Da
• NCBI Official Full Name: Homo sapiens mutY homolog (E. coli), mRNA
• NCBI Official Synonym Full Names: mutY DNA glycosylase
• NCBI Official Symbol: MUTYH
• NCBI Official Synonym Symbols: MYH
• NCBI Protein Information: adenine DNA glycosylase

NCBI Description

This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. Mutations in this gene result in heritable predisposition to colon and stomach cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]