Rat Bone Morphogenetic Protein 4 (BMP4) CLIA Kit | BMP4 clia kit

Rat Bone Morphogenetic Protein 4 (BMP4) CLIA Kit

Cat. Num. AAA2708466

• Gene Names: Bmp4; BOMPR4A
• Reactivity: Rat
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Bone Morphogenetic Protein 4 (BMP4); Rat Bone Morphogenetic Protein 4 (BMP4) CLIA Kit; Bone Morphogenetic Protein 4; BMP2B; BMP2-B; BMP2B1; ZYME; DVR4; Bone morphogenetic protein 2B; BMP4 clia kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 4 (BMP4).; No significant cross-reactivity or interference between Bone Morphogenetic Protein 4 (BMP4) and analogues was observed.
• Sequence Length: 408

• Applicable Applications for BMP4 clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 13.7-10000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 5.1pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 4 (BMP4) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 4 (BMP4) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 4 (BMP4). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 4 (BMP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Bone Morphogenetic Protein 4 (BMP4) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 543902
• NCBI GeneID: 25296
• UniProt Accession #: Q06826
• NCBI Official Full Name: Bone morphogenetic protein 4
• NCBI Official Synonym Full Names: bone morphogenetic protein 4
• NCBI Official Symbol: Bmp4
• NCBI Official Synonym Symbols: BOMPR4A
• NCBI Protein Information: bone morphogenetic protein 4
• UniProt Protein Name: Bone morphogenetic protein 4
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: Bmp4
• UniProt Synonym Gene Names: Bmp-4; Dvr-4; BMP-4; BMP-2B

NCBI Description

plays a role in induction of cell proliferation [RGD, Feb 2006]

Uniprot Description

Induces cartilage and bone formation. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction ().