Typical Testing Data/Standard Curve (for reference only)
Rabbit NOS1/nNOS ELISA Kit | NOS1/nNOS elisa kit
Rabbit NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to NOS1/nNOS. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for NOS1/nNOS and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain NOS1/nNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of NOS1/nNOS. You can calculate the concentration of NOS1/nNOS in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]
Uniprot Description
nNOS: nitric oxide synthase 1, neuronal form. Produces nitric oxide which is a messenger molecule with diverse functions and displays many properties of a neurotransmitter in the brain and peripheral nervous system. May be an effector enzyme for the dystrophin complex. Four alternatively spliced isoforms have been described.
Protein type: EC 1.14.13.39; Oxidoreductase; Amino Acid Metabolism - arginine and proline
Chromosomal Location of Human Ortholog: 12q24.22
Cellular Component: photoreceptor inner segment; cytoskeleton; protein complex; sarcoplasmic reticulum; mitochondrion; perinuclear region of cytoplasm; cytoplasm; dendritic spine; synapse; cytosol; sarcolemma; lipid raft
Molecular Function: calmodulin binding; protein binding; FAD binding; sodium channel regulator activity; FMN binding; nitric-oxide synthase activity; NADPH-hemoprotein reductase activity; iron ion binding; heme binding; oxidoreductase activity; NADP binding; cadmium ion binding
Biological Process: interaction with host; negative regulation of serotonin uptake; negative regulation of calcium ion transport; striated muscle contraction; negative regulation of potassium ion transport; positive regulation of transcription, DNA-dependent; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; positive regulation of vasodilation; response to heat; negative regulation of hydrolase activity; exogenous drug catabolic process; response to hypoxia; peptidyl-cysteine S-nitrosylation; arginine catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone acetylation; negative regulation of blood pressure; blood coagulation; nitric oxide biosynthetic process; multicellular organismal response to stress; nitric oxide mediated signal transduction; myoblast fusion; neurotransmitter biosynthetic process
Disease: Pyloric Stenosis, Infantile Hypertrophic, 1
Research Articles on NOS1/nNOS
Similar Products
Product Notes
The Rabbit NOS1/nNOS nos1 (Catalog #AAA2513801) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2513801 ELISA Kit recognizes Rabbit NOS1/nNOS. It is sometimes possible for the material contained within the vial of "NOS1/nNOS, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
