Rabbit GATA1 Polyclonal Antibody | anti-GATA1 antibody

GATA1 (Phospho-Ser142) Antibody

Cat. Num. AAA9503036

• Gene Names: GATA1; GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: GATA1; Polyclonal Antibody; GATA1 (Phospho-Ser142) Antibody; Erythroid transcription factor; Eryf1; GATA-binding factor 1; GATA-1; GF-1; NF-E1 DNA-binding protein; ERYF1; GF1; anti-GATA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: GATA1 (Phospho-Ser142) Antibody detects endogenous levels of GATA1 only when phosphorylated at Ser142.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 330

• Applicable Applications for anti-GATA1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:5000
• Target Modification: Phospho
• Modification Sites: H:S142; M:S142; R:S142
• Immunogen: The antiserum was produced against synthesized peptide derived from human GATA1 around the phosphorylation site of Ser142.
• Immunogen Range: 109-158
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GATA1 (Phospho-Ser142) Antibody. The picture on the right is blocked with the phospho peptide.)

Western Blot (WB)

(Western blot analysis of lysates from K562 cells, using GATA1 (Phospho-Ser142) Antibody. The lane on the right is blocked with the phospho peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 4503925
• NCBI GeneID: 2623
• NCBI Accession #: NP_002040.1
• NCBI GenBank Nucleotide #: NM_002049.3
• UniProt Accession #: P15976; Q96GB8
• Molecular Weight: 42 kDa
• NCBI Official Full Name: erythroid transcription factor
• NCBI Official Synonym Full Names: GATA binding protein 1
• NCBI Official Symbol: GATA1
• NCBI Official Synonym Symbols: GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1
• NCBI Protein Information: erythroid transcription factor
• UniProt Protein Name: Erythroid transcription factor
• Protein Family: GATA transcription factor
• UniProt Gene Name: GATA1
• UniProt Synonym Gene Names: ERYF1; GF1; GATA-1; GF-1

NCBI Description

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed:24245781).