Rabbit anti-Human NOD2 Polyclonal Antibody | anti-Nod2 antibody

NOD2, NT (CARD15, Nucleotide-binding Oligomerization Domain-containing Protein 2, Caspase Recruitment Domain-containing Protein 15, Inflammatory Bowel Disease Protein 1, IBD1)

Cat. Num. AAA643445

• Gene Names: Nod2; CD; ACUG; BLAU; IBD1; Nlrc2; Card15; F830032C23Rik
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: NOD2; Polyclonal Antibody; NT (CARD15; Nucleotide-binding Oligomerization Domain-containing Protein 2; Caspase Recruitment Domain-containing Protein 15; Inflammatory Bowel Disease Protein 1; IBD1); Anti -NOD2; anti-Nod2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human NOD2.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-Nod2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.; Dilution: Western Blot: 2-4ug/ml; Immunohistochemistry (Formalin-fixed, paraffin-embedded): 3ug/ml; Immunocytochemistry: 5ug/ml
• Immunogen: Synthetic peptide corresponding to 16aa from human NOD2 at N-terminal (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Small Intestine (formalin-fixed, paraffin-embedded) stained with NOD2 antibody at 3ug/ml followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Western Blot (WB)

(Western blot analysis of NOD2 in Jurkat cell lysate with NOD2 antibody at (A) 1, (B) 2 and (C) 4ug/ml.)

Related Product Information for anti-Nod2 antibody

NOD2 is an intracellular protein of 100kD, which belongs to the NOD (nucleotide binding oligomerisation domain) family. The NOD family comprises at present more than 20 different mammalian NOD-LRR (leucine-rich repeat) proteins, which are involved in the regulation of apoptosis and the immune response. NOD2 has been shown to be constitutively or inducibly expressed in monocytes, macrophages, T and B cells, dendritic cells, as well as intestinal epithelial cells. A specific motif of peptidoglycan (PGN), muramyl dipeptide (MDP), has been identified as the sole ligand of NOD2. NOD2 and Toll-like receptors (TLR) are emerging as key mediators of innate host defence in the intestinal mucosa, crucially involved in maintaining mucosal and commensal homeostasis. Mutations in the gene encoding NOD2 are a major susceptibility factor underlying a significant subgroup of patients with Crohn's disease.

Product Categories/Family for anti-Nod2 antibody

Antibodies; Abs to Apoptosis; Caspase

NCBI and Uniprot Product Information

• NCBI GI #: 30017199
• NCBI GeneID: 257632
• UniProt Accession #: Q8K3Z0
• Molecular Weight: 113,562 Da
• NCBI Official Full Name: NOD2
• NCBI Official Synonym Full Names: nucleotide-binding oligomerization domain containing 2
• NCBI Official Symbol: Nod2
• NCBI Official Synonym Symbols: CD; ACUG; BLAU; IBD1; Nlrc2; Card15; F830032C23Rik
• NCBI Protein Information: nucleotide-binding oligomerization domain-containing protein 2; caspase recruitment domain family, member 15; caspase recruitment domain-containing protein 15
• UniProt Protein Name: Nucleotide-binding oligomerization domain-containing protein 2
• Protein Family: Nucleotide-binding oligomerization domain-containing protein
• UniProt Gene Name: Nod2
• UniProt Synonym Gene Names: Card15
• UniProt Entry Name: NOD2_MOUSE

Uniprot Description

NOD2: Induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK- gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Defects in NOD2 are the cause of Blau syndrome (BS). BS is a rare autosomal dominant disorder characterized by early-onset granulomatous arthritis, uveitis and skin rash. Defects in NOD2 are a cause of susceptibility to inflammatory bowel disease type 1 (IBD1). IBD1 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Defects in NOD2 are the cause of sarcoidosis early-onset (EOS). EOS is a form of sarcoidosis manifesting in children younger than 4 years of age. Sarcoidosis is an idiopathic, systemic, inflammatory disease characterized by the formation of immune granulomas in involved organs. Granulomas predominantly invade the lungs and the lymphatic system, but also skin, liver, spleen, eyes and other organs may be involved. Early- onset sarcoidosis is quite rare and has a distinct triad of skin, joint and eye disorders, without apparent pulmonary involvement. Compared with an asymptomatic and sometimes naturally disappearing course of the disease in older children, early-onset sarcoidosis is progressive and in many cases causes severe complications, such as blindness, joint destruction and visceral involvement. 2 isoforms of the human protein are produced by alternative initiation.

Cellular Component: signalosome; cytoskeleton; protein complex; cell surface; cytoplasm; plasma membrane; intracellular; cytosol; vesicle

Molecular Function: endodeoxyribonuclease activity, producing 5'-phosphomonoesters; protein binding; peptidoglycan binding; enzyme binding; CARD domain binding; nucleotide binding; protein kinase binding; ATP binding; muramyl dipeptide binding

Biological Process: positive regulation of dendritic cell antigen processing and presentation; maintenance of gastrointestinal epithelium; response to lipopolysaccharide; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-10 production; negative regulation of interleukin-2 production; activation of NF-kappaB transcription factor; regulation of apoptosis; positive regulation of phagocytosis; detection of muramyl dipeptide; cytokine production during immune response; detection of biotic stimulus; positive regulation of oxidoreductase activity; positive regulation of interleukin-17 production; negative regulation of interleukin-12 production; positive regulation of T-helper 2 type immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of dendritic cell cytokine production; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; protein oligomerization; negative regulation of interleukin-18 production; positive regulation of peptidyl-tyrosine phosphorylation; defense response to Gram-positive bacterium; innate immune response in mucosa; response to muramyl dipeptide; inhibition of NF-kappaB transcription factor; positive regulation of B cell activation; defense response to bacterium; positive regulation of transcription from RNA polymerase II promoter; negative regulation of T cell mediated immunity; positive regulation of epithelial cell proliferation; positive regulation of nitric-oxide synthase biosynthetic process; activation of immune response; detection of bacterium; positive regulation of interleukin-12 production; positive regulation of JNK cascade; defense response; signal transduction; positive regulation of stress-activated MAPK cascade; response to exogenous dsRNA; positive regulation of MAPKKK cascade; negative regulation of interferon-gamma production; response to peptidoglycan; positive regulation of cell proliferation; positive regulation of innate immune response; negative regulation of inflammatory response to antigenic stimulus; inflammatory response; positive regulation of biosynthetic process of antibacterial peptides active against Gram-positive bacteria; positive regulation of Notch signaling pathway; positive regulation of humoral immune response mediated by circulating immunoglobulin; immune system process; positive regulation of antibacterial peptide biosynthetic process; negative regulation of toll-like receptor 2 signaling pathway; positive regulation of phosphoinositide 3-kinase activity; negative regulation of tumor necrosis factor production; immunoglobulin production during immune response; positive regulation of interleukin-1 beta production; positive regulation of protein ubiquitination; regulation of inflammatory response; innate immune response